相关实验视频

Updated: May 24, 2025

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

单胺通过mTOR/P70S6K通路在慢性结肠炎中抑制表皮层-介质细胞转换

Yuanfan Chen^1,2,3, Jiaying Liu¹, Shaowen Zhong¹

¹School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

Frontiers in pharmacology

|March 5, 2025

概括

来自传统中医药的单烯,通过阻断表皮层-介质细胞转换 (EMT) 来有效抑制慢性结肠炎中的肠纤维化. 这种作用与mTOR/P70S6K通路有关,为结肠炎相关纤维化提供了潜在的治疗策略.

科学领域:

胃肠病学胃肠病学
药理学药理学是指药理学的学科.
细胞生物学细胞生物学

背景情况:

慢性结肠炎通过表皮细胞-介质细胞过渡 (EMT) 构成肠道纤维化的风险.
来自Morinda officinalis的单素 (MON) 可能改善性结肠炎,但其机制和对纤维化的影响尚不清楚.
研究MON对慢性结肠炎EMT的影响对于了解其治疗潜力至关重要.

研究的目的:

在慢性结肠炎中研究单素对上皮层-介质细胞过渡 (EMT) 的作用.
阐明单胺在结肠炎相关纤维化中的作用的潜在分子机制.

主要方法:

一个慢性结肠炎的小鼠模型被使用德克斯硫酸盐 (DSS) 诱导.
在体内和体外使用TGF-β1诱导的IEC-6细胞评估了单烯对EMT的抑制作用.
分子对接,MD模拟,CETSA和DARTS测定确定了单质素的目标为mTOR.

主要成果:

单素显著改善了结肠损伤,并抑制了DSS诱导的大肠炎中的EMT标志物.
单胺直接向mTOR,抑制其酸化和下游信号在细胞和动物模型.
作为对单胺治疗的反应,观察到自活动的增加.

结论:

单胺在慢性结肠炎中有效抑制EMT,这表明它在预防肠道纤维化方面发挥了作用.
单素的治疗作用通过mTOR/P70S6K信号通路进行介导.
单烯为治疗慢性结肠炎相关肠纤维化提供了一个有前途的治疗药物.

关键词:

在 DSS 中使用 DSS.慢性结肠炎是一种慢性结肠炎.表皮介质介质的过渡.mTOR/P70S6KK的使用情况.单种类型中的一种.

更多相关视频

Induction and Analysis of Epithelial to Mesenchymal Transition

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Published on: September 12, 2019

相关概念视频

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...