在芯片上的肠道微生物-皮肤轴,用于复制炎症交叉通话
Byungho Ko1, Jimin Son1, Jong In Won1
1Department of Chemical Engineering, Hongik University, Seoul, 04066, Republic of Korea. jhsung22@hongik.ac.kr.
Lab on a chip
|March 5, 2025
概括
一个新的肠道微生物皮肤芯片模拟了肠道皮肤轴,揭示了肠道细菌如何影响皮肤健康. 这个平台显示Lactobacillus rhamnosus GG通过加强肠道屏障来保护皮肤.
科学领域:
- 微流体学和芯片上器官技术
- 微生物组研究 微生物组研究
- 皮肤病学和皮肤生物学
背景情况:
- 肠-皮肤轴将肠道微生物群与皮肤健康和疾病联系起来.
- 由于缺乏合适的实验模型,研究肠-皮肤相互作用是具有挑战性的.
- 了解这些机制对于开发用于皮肤疾病的新型治疗策略至关重要.
研究的目的:
- 开发和验证一种新的微流体平台,即肠道微生物皮肤 (GMS) 芯片.
- 模拟肠道微生物群,肠道细胞和皮肤细胞之间的相互作用.
- 研究肠道微生物在调节皮肤健康和疾病中的作用.
主要方法:
- 开发一种微流体GMS芯片,使Caco-2肠道细胞,HEKa皮肤细胞和肠道微生物能够共同培养.
- 在芯片内复制生理条件,包括流体连接和氧气梯度.
- 使用硫酸 (DSS) 和脂多糖 (LPS) 诱导肠道损伤,以评估对皮肤细胞的影响.
主要成果:
- 该GMS芯片成功支持肠道和皮肤细胞与肠道细菌 (大肠杆菌,LGG) 的共同培养.
- 肠道屏障的破坏导致皮肤细胞活力下降.
- 预处理用乳杆菌rhamnosus GG (LGG) 通过增强肠道屏障功能来保护皮肤细胞.
结论:
- GMS芯片是一个经过验证的平台,用于研究肠-皮肤轴和微生物组-肠-皮肤相互作用.
- 这个模型证明了肠道微生物群对皮肤健康的直接影响.
- 这些发现凸显了针对肠道微生物群进行皮肤疾病管理的潜力.
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