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在Sideroxydans lithotrophicus ES-1中,微生物磁铁氧化通过MtoAB蛋白-多重体细胞染色体复合体

Jessica L Keffer¹, Nanqing Zhou², Danielle D Rushworth¹

¹Department of Earth Sciences, University of Delaware, Newark, Delaware, USA.

Applied and environmental microbiology

|March 5, 2025

概括

像Sideroxydans lithotrophicus ES-1这样的氧化铁的细菌可以使用固体磁铁作为能量来源,使用不同的细胞外电子转移通路. 这一发现扩大了我们对微生物代谢及其在地质过程中的作用的理解.

科学领域:

微生物学微生物学
地质化学地质化学
生物化学生物化学

背景情况:

大多数地球上的铁是矿物结合的,对微生物利用固体矿物质作为电子捐赠者的理解有限.
氧化铁的细菌具有不同的铁氧化机制,可能与不同的Fe (II) 源有关.

研究的目的:

调查Sideroxydans lithotrophicus ES-1是否可以利用固体磁铁作为电子捐赠体.
为了确定参与固体矿物质氧化的特定蛋白质和代谢途径.

主要方法:

在溶解的Fe (II) - 酸盐和磁铁上培养Sideroxydans lithotrophicus ES-1.
定量蛋白质组分析以比较不同生长条件下的蛋白质表达.

主要成果:

在固体磁铁上,Sideroxydans lithotrophicus ES-1 显示出生长,并将其用作电子捐赠体.
蛋白质组学揭示了不同的蛋白质表达模式,在固体磁铁氧化过程中,含有MtoA和cupredoxin的蛋白质特别升级.
在溶解和固体基板上表达Cyc2铁氧化酶.

结论:

与溶解的Fe (II) 相比,Sideroxydans lithotrophicus ES-1使用额外的细胞外电子转移途径进行固体矿物质氧化.
Mto通路和特定的铜素可能参与固体铁矿物质的氧化.
这项研究扩大了对微生物铁氧化及其生态影响的知识.

关键词:

在Gallionellaceae的植物中.在Sideroxydans中铁氧化剂是一种铁氧化剂.磁铁的磁铁是磁性质的磁铁.微生物-矿物相互作用多重体细胞染色体的多重体细胞染色体

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