在Helicobacter pylori中使用下一代测序与向丰富技术的耐药性和病毒组确定
Léo Gillet1, Lucie Bénéjat1, Quentin Jehanne1
1CHU de Bordeaux, CNR des Campylobacters et des Hélicobacters, Bordeaux, France.
Microbiology spectrum
|March 5, 2025
概括
一种新的下一代测序 (NGS) 方法可以从胃活检直接快速评估Helicobacter pylori的抗生素耐药性和毒性. 这种方法有助于选择针对H. pylori感染的向疗法.
科学领域:
- 微生物学与传染病的研究
- 基因组学和分子诊断的研究.
背景情况:
- 杆菌感染影响全球超过50%的人口,由于耐药性数据有限,通常使用宽谱抗生素治疗.
- 杆菌的脆弱性使得传统的培养方法对准确的诊断和耐药性分析具有挑战性.
- 下一代测序 (NGS) 提供了直接评估细菌遗传特征的潜力,包括抗微生物耐药性和毒性因素.
研究的目的:
- 开发和验证一种基于NGS的新方法,用于从胃活检中直接分析H. pylori抗体和病毒体.
- 为了快速识别与抗生素耐药性和H. pylori关键毒性因素相关的遗传标记.
主要方法:
- 使用DNA丰富的向测序方法在19个阳性H. pylori的胃活检样本上使用.
- 使用Agilent SureSelectXT协议与自定义诱库,然后在Illumina iSeq 100平台上进行测序.
- 用RNA探针测序病毒性,耐药性和分子类型基因.
主要成果:
- 开发的NGS方法实现了H. pylori大约1.8e5 CFU/mL的检测极限.
- 准确识别与宏化物和levofloxacin耐药性相关的突变被证明.
- MLST 基因组,cagA 检测和 vacA 基因定型结果与传统方法一致,验证了丰富策略.
结论:
- 这种基于缩的新型NGS方法提供了一种快速有效的手段,可以直接从胃活检中评估H. pylori抗体和病毒体.
- 这种方法促进了知情的,有针对性的抗微生物治疗选择,改善了H. pylori根除率和患者的治疗结果.
- 这种方法比传统的培养技术有了显著的进步,解决了H. pylori感染临床诊断的局限性.
更多相关视频
08:56Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
10.9K
06:02Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
Published on: September 13, 2018
6.8K
相关概念视频
03:00Next-generation Sequencing
87.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.9K
01:16Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
2.5K
Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
2.5K
01:29Modern Molecular Taxonomy
836
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
836
01:26Human Virome
47
The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible...
47