胚胎液衍生的介质干细胞中的复制性衰老及其对其免疫调节性质的影响
Elham Zare1,2, Elham Sadat Hosseini3, Faezeh Sadat Azad2
1Medical Genetics and Molecular Medicine Department, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Histochemistry and cell biology
|March 5, 2025
概括
复制性衰老并不会显著损害胎液衍生的介质干细胞 (AF-MSCs) 的免疫调节特性. 衰老的AF-MSC保留了治疗潜力,尽管长时间培养,但在关键免疫基因表达中表现出弹性.
科学领域:
- 干细胞生物学 干细胞生物学
- 免疫学 免疫学 免疫学
- 再生医学是一种再生医学.
背景情况:
- 介质干细胞 (MSC) 对于再生医学至关重要,但它们的临床使用受到体外扩张过程中复制性衰老的限制.
- 衰老对MSC的免疫调节能力的影响,特别是那些来自羊水的MSC (AF-MSC),还不太清楚.
研究的目的:
- 研究复制性衰老对AF-MSCs免疫调节基因表达的影响.
- 评估AF-MSCs在长时间培养和冷保存后的分化潜力和免疫调节稳定性.
主要方法:
- AF-MSCs来自第一季度的胎液,并通过流细胞计测定其特征.
- 评估了骨质原和脂肪原的差异化潜力.
- 免疫调节基因表达 (TGFβ,IL-10,IDO,VCAM-1,FASL,IL-6,COX1,HLA-G) 在增殖和衰老的AF-MSC之间进行了比较.
- 评估了细胞增殖标志物 (FOXM1,B-MYB).
主要成果:
- 衰老的AF-MSC在冷保存后保持了脂肪原分化能力.
- 关键的免疫调节基因 (TGFβ,IL-10,IDO,VCAM-1) 在增殖细胞和衰老细胞之间没有显著差异.
- 衰老细胞表现出FASL的下调和IL-6,COX1和HLA-G的上调.
- 细胞增殖标志物FOXM1和B-MYB在衰老细胞中显著下调.
结论:
- 尽管复制性衰老,但AF-MSCs在其免疫调节特性中表现出弹性.
- 衰老并不废除AF-MSCs的潜在治疗应用.
- 这些发现支持在临床环境中使用AF-MSC,即使在扩展体外培养后也是如此.
相关概念视频
01:19Mesenchymal Stem Cells
4.6K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.6K
01:01Regulation of Hematopoietic Stem Cells
3.1K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.1K
01:33Adult Stem Cells
27.8K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
27.8K
01:22iPS Cell Differentiation
2.6K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.6K
02:15Replicative Cell Senescence
3.6K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.6K
01:26Stem Cell Niche
5.0K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.0K