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Evaluation of the Storage Stability of Extracellular Vesicles
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从细胞单层和球状体中获得的细胞外囊泡之间的促凝活性的比较
Araci M R Rondon1,2, Sophie Featherby3, Tainá Gomes4
1Einthoven Laboratory for Vascular and Regenerative Medicine, Division of Thrombosis and Hemostasis, Department of Internal Medicine, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands. a.m.da_rocha_rondon@lumc.nl.
Journal of thrombosis and thrombolysis
|March 5, 2025
概括
二维 (单层) 细胞培养物比三维 (球形) 培养物释放的细胞外囊泡 (EV) 和组织因子 (TF) 显著更多. 这种差异影响了癌症相关血栓瘤研究模型.
科学领域:
- 生物化学 生物化学
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
背景情况:
- 组织因子 (TF) 是血液凝固的关键启动因子.
- 癌细胞通过细胞外囊泡 (EV) 释放的TF与血栓形成和瘤进展有关.
- 了解不同癌症模型中的TF释放机制至关重要.
研究的目的:
- 为了比较2D (单层) 与3D (球形) 模型中培养的癌细胞中EV和TF的释放.
- 研究与癌症相关纤维细胞 (CAFs) 共同培养对EV和TF释放的影响.
- 评估蛋白酶激活受体2 (PAR-2) 激活对EV和TF释放的影响.
主要方法:
- Hs578t人类乳腺癌细胞和CAFs被培养成单层和球形.
- 使用脂氨酸度和TF-ELISA测量了EV和TF度.
- 纳米粒子跟踪分析确定了EV大小和度.
- 凝血试验评估了TF的前凝血活性.
主要成果:
- 单层培养物释放的EV数量是球形培养物的7倍,TF抗原的11倍.
- PAR-2激活在单层中显著增加了EV和TF释放,但在球体中没有.
- Hs578t/CAF球体在PAR-2激活时显示了EV和TF释放的轻微增加.
结论:
- 单层细胞培养与球形培养相比,产生更多的EV和相关TF.
- 癌细胞培养方法 (2D与3D) 显著影响EV和TF释放动态.
- 选择适当的细胞模型对于研究与癌症相关的血栓形成至关重要.
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