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Mohd Shoeb Alam¹, Abhijeet Dhiman¹, Tanu Bhardwaj²

¹Department of Biotechnology, All India Institute of Medical Sciences, New Delhi 110029, India.

ACS infectious diseases

|March 5, 2025

概括

新的DNA体提供了一个高度特定和准确的诊断工具,用于Plasmodium vivax疟疾. 这一突破有望为检测疟疾感染提供更容易获得和更可靠的方法,特别是在疾病负担较高的地区.

科学领域:

寄生虫学的寄生虫学
分子诊断学分子诊断学
生物技术是生物技术.

背景情况:

疟疾仍然是一个重大的全球健康负担,特别是印度的Plasmodium vivax疟疾.
目前用于P.vivax疟疾的诊断方法往往受到高成本,复杂性或精度低的困扰.
对于P.vivax疟疾的特定和可靠的诊断试验有着迫切的需要.

研究的目的:

开发具有高特异性的新型DNA吸附体,用于Plasmodium vivax富抗原 (PvTRAg).
为了评估这些体的诊断潜力,使用体连接的固定吸附剂试验 (ALISA).
为了建立一个新的aptamer-based诊断测试的基础,用于P. vivax疟疾.

主要方法:

通过指数式丰富对联体的系统进化 (SELEX) 用于识别针对PvTRAg.的特定体.
选择了Apt_14和Apt_16两种高亲和度的阿胺,分别具有1.9nM和1.2nM的解离常数 (KD).
在临床验证中,APTAMER在ALISA格式中用于检测P.vivax疟疾感染.

主要成果:

Apt_14和Apt_16对PvTRAg具有很高的亲和力,检测极限在2.5nM.
临床验证显示高特异性 (100%) 和良好的敏感性 (Apt_14的96%和Apt_16的84%).
体表现出最小的与其他抗原的交叉反应性,证实了它们对PvTRAg.g的选择性.

结论:

DNA 体是用于准确诊断Plasmodium vivax疟疾的有效工具.
开发的体和ALISA方法对一种新的,特定的诊断试验有很大的希望.
这项研究为改善疟疾诊断铺平了道路,特别是对于P. vivax感染.

关键词:

这是一种活性菌 (Plasmodium vivax).这就是PvTRAgg.一个aptamer的应用程序.诊断诊断诊断诊断诊断诊断疟疾疟疾是一种疾病.

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