协议重建一个体外3D全厚皮肤等效模型与原I在6和12井插入器中的原I
Khek-Chian Tham1, Seong Soo Lim1, Carine Bonnard2
1A∗STAR Skin Research Labs (A∗SRL), Agency for Science, Technology and Research (A∗STAR), 11 Mandalay Road, #17-01 Clinical Sciences Building, Singapore 308232, Singapore.
STAR protocols
|March 5, 2025
概括
研究人员使用纤维细胞,角质细胞和原蛋白开发了一种3D全厚重建的人体皮肤模型. 这种先进的体外模型模仿了皮肤.
科学领域:
- 组织工程是组织工程.
- 皮肤病学 皮肤病学
- 生物技术是生物技术.
背景情况:
- 二维细胞培养缺乏生理相关性.
- 3D全厚重建的人体皮肤模型提供了增强的差异化.
- 在体外模型对于皮肤研究至关重要.
研究的目的:
- 介绍一个详细的协议,用于重建一个3D人类皮肤模型.
- 为了证明使用奇托来防止组织收缩.
- 提供一个生理上相关的体外皮肤模型.
主要方法:
- 人体纤维细胞和角质细胞的细胞扩张.
- 使用鼠尾原蛋白造细胞和非细胞层 I.
- 空气提升培养技术和化.
- 在12井插入器中应用奇托桑以抑制收缩.
主要成果:
- 成功重建了一个3D全厚人体皮肤模型.
- 奇托桑在预防组织收缩方面已证明有效.
- 该模型表现出与体内皮肤相关的差异化特征.
结论:
- 该协议可以创建一个生理学上相关的3D人体皮肤模型.
- 酸盐是3D皮肤培养中控制组织收缩的有效药物.
- 这种3D皮肤模型是体外皮肤研究和开发的宝贵工具.
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