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相关概念视频

Actin Treadmilling01:18

Actin Treadmilling

7.9K
Actin filaments undergo polymerization and depolymerization from either end. The polymerization and depolymerization rates depend on the cytosolic concentration of free G-actins. The polymerization rate is generally higher at the plus or barbed end, while the depolymerization rate is higher at the minus or pointed end. At a steady state, critical concentration describes the concentration of free G-actin monomers at which the polymerization rate at the plus end is equal to that of the...
7.9K
Actin Polymerization01:42

Actin Polymerization

6.3K
Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
6.3K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

5.1K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.1K
Generation of Straight or Branched Actin Filaments01:14

Generation of Straight or Branched Actin Filaments

2.8K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.8K
Introduction to Actin01:26

Introduction to Actin

4.8K
Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution.  Actin coding genes are conserved within species and across...
4.8K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

5.8K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
5.8K

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相关实验视频

Updated: May 24, 2025

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
09:09

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

9.6K

对于行为动态的时空特征学习.

Siddhartha Saha1, Qixin Yang2, Wolfgang Losert2

  • 1Department of Physics and Astronomy, Rutgers University, Piscataway, New Jersey, United States of America.

PloS one
|March 5, 2025
PubMed
概括
此摘要是机器生成的。

机器学习可以从actin波视频中预测Dictyostelium discoideum细胞微环境. 分析actin动力学揭示了纳米拓和电场对细胞迁移的影响.

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Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
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Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

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Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance
07:53

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

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相关实验视频

Last Updated: May 24, 2025

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
09:09

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

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Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

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Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance
07:53

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

Published on: October 21, 2021

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科学领域:

  • 细胞和分子生物学 细胞和分子生物学
  • 生物物理学的生物物理.
  • 计算生物学 计算生物学

背景情况:

  • 迪西奥斯蒂 (Dictyostelium discoideum) 是研究细胞移动性和模式形成的模型生物.
  • 细胞迁移依赖于actin细胞骨动力学,对微环境线索敏感,如刚性,地形和电场.
  • 了解微环境因素如何影响细胞行为对于生物研究至关重要.

研究的目的:

  • 为了研究机器学习是否可以从Dictyostelium discoideum中的actin波视频中推断微环境条件 (电场,纳米拓).
  • 识别与特定微环境特征相关的行为波的视觉特征.
  • 开发用于分析细胞动态响应物理线索的计算方法.

主要方法:

  • 使用了三种机器学习技术:字典学习,散射变换和光流.
  • 分析了Dictyostelium discoideum的视频显微镜数据.
  • 开发了框架对框架的预测模型,以根据actin波模式对微环境类型进行分类.

主要成果:

  • 词典学习和散射转换通过分析静态图像特征,有效地基于纳米拓学分类细胞.
  • 光流,通过跟踪稳定的细胞特征随着时间的推移,证明有效预测外部电场的存在.
  • 这项研究表明,不同的actin波模式与不同的微环境条件相关.

结论:

  • 动因波的机器学习分析提供了一个强大的方法来推断微环境属性.
  • 特定的机器学习方法更适合识别不同类型的物理线索.
  • 这种计算框架可用于使用视频显微镜研究各种生物系统中的集体细胞动力学.