在来自Candida auris的D-arabinose脱酶中对基质识别循环灵活性进行结构分析
Meng Dan1, Zhang Jie1, Bai Xue2
1Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian, 116600, Liaoning, China; Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, China.
Biochemical and biophysical research communications
|March 5, 2025
概括
黄虫使用d-arabinose脱酶 (CaAldO) 产生抗氧化剂,帮助其生存. 了解 CaAldOO 的意思
科学领域:
- 菌类学 菌类学是指菌类学.
- 结构生物学 结构生物学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 黄是一种多药耐药的真菌病原体,引起了全球卫生方面的关注.
- 抗氧化应激对C. auris的生存和毒性至关重要.
- 在C. auris.中,d-arabinose脱酶 (CaAldO) 对于合成抗氧化剂d-erythroascorbic acid (EASC) 是至关重要的.
研究的目的:
- 为了确定C. auris d-arabinose脱酶 (CaAldO) 的高分辨率晶体结构.
- 了解CaAldO在氧化应激抵抗中的作用的结构基础.
- 将CaAldO与其同类进行比较,以了解抗真菌策略.
主要方法:
- 在1.95 Å时对CaAldO的高分辨率晶体结构的确定.
- 对辅助因子结合口袋和基板识别环的分析.
- 与同类酶的结构比较,如Scara1.
主要成果:
- CaAldO的晶体结构显示了一个TIM-桶折叠,由四个循环区域组成的辅因子结合口袋.
- 基板结合口袋中的灵活环A和C可以促进构造变化.
- 在CaAldO和ScAra1之间观察到显著的结构差异,特别是在基质识别和辅因子结合口袋中.
结论:
- CaAldO的结构灵活性有助于其在抗氧化应激的功能.
- 与同类相比,CaAldO的独特结构特征为新型抗真菌疗法提供了潜在的标.
- 了解CaAldO的结构是制定针对新出现的病原体Candida auris.的战略的关键.
相关概念视频
00:49Ligand Binding and Linkage
4.7K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.7K
01:19Allosteric Proteins-ATCase
5.6K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.6K