适应单分子实时 (SMRT) 序列技术用于C型肝炎病毒基因组测序和抵抗相关替代物的识别
Cui Zhang1, Pei Liu1, Jian Li1
1National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
Virology
|March 5, 2025
概括
在10%的值下,PacBio测序能够准确地检测出C型肝炎病毒 (HCV) 耐药性相关替代物 (RAS),为大规模人口研究提供了NGS的成本效益替代方案.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 分子诊断学 分子诊断学
背景情况:
- 肝炎C病毒 (HCV) 耐药性相关替代物 (RAS) 对直接作用抗病毒 (DAA) 治疗疗效至关重要.
- 准确检测HCV RAS对于有效的HCV治疗策略至关重要.
- 帕克生物测序为全面的遗传分析提供了长读能力.
研究的目的:
- 与桑格测序相比,评估PacBio测序用于检测HCV RASs的准确性.
- 评估PacBio测序与下一代测序 (NGS) 对HCV RAS检测的成本效益.
- 为了确定PacBio测序分析HCV RASs的最佳值.
主要方法:
- 在28个HCVRNA阳性标本上进行了PacBio测序,针对NS3到NS5B区域.
- 序列数据使用lima,pbmm2和quasitools软件进行处理.
- 分析涉及不同的频率值:1%,5%,10%和15%.
主要成果:
- 通过PacBio测序确定了高频率,罕见和低值的HCV RAS.
- 在10%值的结果表明与桑格测序的可比性.
- PacBio 测序成本 (571 CNY/标本) 比 NGS (1000 CNY/标本) 显著低.
结论:
- 对于对HCV RASs的PacBio测序分析,建议使用10%的值.
- PacBio 测序为大规模 HCV RAS 检测提供了一个有希望的,具有成本效益的工具.
- 这种方法支持加强对C型肝炎的监测和治疗监测.
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