油性酵母Rhodosporidium toruloides是为生物质水解酸衍生 (E) -α-bisabolene生产而设计的
Paul A Adamczyk1, Hee Jin Hwang1, Ta-Hsuan Chang2
1Agile Biofoundry, Emeryville, CA, USA; Sandia National Laboratories, Livermore, CA, USA.
Metabolic engineering
|March 5, 2025
概括
这项研究使酵母Rhodosporidium toruloides产生 (E) -α-bisabolene,达到创纪录的高位和生产率. 代谢途径和培养条件的优化是增强烯生物合成的关键.
科学领域:
- 生物技术和合成生物学
- 代谢工程是代谢工程.
- 酵母发酵 酵母发酵的方法
背景情况:
- 油性酵母Rhodosporidium toruloides是生物产品的一个有前途的平台,因为它的脂质积累和耐受生物质抑制剂.
- 之前的工作在这种酵母中建立了 (E) -α-bisabolene生产,但为了工业可行性需要进一步优化.
研究的目的:
- 通过改造美酸盐路径并优化发酵条件,增强Rhodosporidium toruloides中的 (E) -α-bisabolene生产.
- 为了识别和克服烯生物合成中的代谢瓶.
主要方法:
- 来自Saccharomyces cerevisiae的完整的美瓦酸途径的代堆叠.
- 代谢和蛋白质组分析以确定代谢瓶.
- 选反抗性美酸盐激酶的检测.
- 优化和减弱竞争的代谢流量.
- 媒体优化,包括的作用.
- 在微型板和生物反应器中使用玉米炉水解剂进行高密度培养.
主要成果:
- 与对照组相比,工程菌株获得了明显更高的 (E) -α-bisabolene标位,通过编码子优化和流量减弱分别提高了6倍和7倍.
- 识别并解决了代谢瓶,通过反耐药的甲酸激酶查,导致15%的标位改善.
- 媒体优化,特别是添加,进一步提高了10%的标位.
- 在使用玉米水解剂在300g/L总糖的微型板中实现了20.8g/L (E) -α-bisabolene的记录标位.
- 2L生物反应器发酵产生了19.3g/L (E) -α-bisabolene,生产率为0.11g/L/h,这是文献中的一个高点.
结论:
- 该研究成功设计了Rhodosporidium toruloides用于高水平的 (E) -α-bisabolene生产,证明了综合代谢工程策略的力量.
- 开发的高性能菌株和优化的工艺代表了从林氏纤维素生物质中可持续生产烯的重大进步.
相关概念视频
01:25Microbes in Beverage Production
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
01:22Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
01:27Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
01:25Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
01:30Production of Pharmaceuticals
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...
01:25Biofuels
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...