在有氧条件下的土壤Neobacillus和Bacillus菌株中分散的酸盐减少
Seohyun Ahn1, Min Cho1, Michael J Sadowsky2
1Division of Biotechnology and Advanced Institute of Environment and Bioscience, Jeonbuk National University, Jeonbuk 54596, Republic of Korea.
Journal of microbiology (Seoul, Korea)
|March 5, 2025
概括
三种土壤细菌菌株进行有氧脱和非仿真性酸盐降解为 (DNRA),挑战了这些过程需要无氧条件的概念. 这一发现突显了它们在土壤循环中的潜在作用.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物地质化学生物地质化学
背景情况:
- 无化和异样化酸盐降解为 (DNRA) 传统上与无氧细菌在无毒条件下利用酸盐 (NO3-) 相关.
- 酸盐减少途径对土壤循环至关重要,影响的可用性和温室气体排放.
研究的目的:
- 为了研究三种土壤细菌菌株 (Neobacillus spp. PS2-9,PS3-12和Bacillus salipaludis PS3-36) 从一个韩国大米田中分离出来.
- 为了确定这些菌株是否可以在有氧条件下进行脱和DNRA.
主要方法:
- 从土壤中分离和识别细菌菌株.
- 全基因组测序以确定参与酸盐减少的功能基因.
- 在不同氧气条件下进行表型和转录分析,以评估酸盐降解途径.
主要成果:
- 这三种选择性无氧菌株都具有脱和DNRA的基因.
- 这些菌株在有氧和无氧条件下减少了酸盐,并在细胞外产生了氨.
- 在有氧条件下观察到脱和DNRA基因 (nirK, nosZ, nirB, nrfA) 的同时转录.
结论:
- 这项研究报告了第一例在有氧条件下在Bacillus或Neobacillus菌株中共存酸盐同化,脱和DNRA的实例.
- 这些发现挑战了这些过程的严格无氧要求.
- 鉴定出的细菌菌株可能会显著影响土壤循环,因为它们具有多功能酸盐代谢.
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