在Lactiplantibacillus plantarum上显示两个新抗原的表面显示
Kamilla Wiull1, Erik Holmgren2, Samuel Svensson3
1Faculty of Chemistry, Biotechnology and Food Science, NMBU - Norwegian University of Life Sciences, Ås, Norway. kamilla.wiull@nmbu.no.
Scientific reports
|March 5, 2025
概括
乳植物菌 (Lactiplantibacillus plantarum) 可以在其表面产生和显示癌症新抗原. 这种针对癌症免疫治疗的新方法可以增强抗原暴露和免疫反应潜力.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
背景情况:
- 乳酸细菌 (LAB) 在治疗性蛋白质输送方面得到了探索.
- 对细菌细胞的抗原定可以防止蛋白质分解,并增强免疫反应.
- 使用瘤特异性新抗原的个性化免疫疗法在癌症研究中显示出前景.
研究的目的:
- 为了研究使用Lactiplantibacillus plantarum的新抗原 (NAG1和ETV6) 的表面显示.
- 评估不同的定方法,以准新抗原到细菌细胞膜和细胞壁.
- 评估定对新抗原生产和表面暴露的影响.
主要方法:
- 在L. plantarum中利用了可诱导的PSIP表达系统.
- 采用四种不同的定策略来准新抗原.
- 分析了使用流细胞计的抗原生产和表面暴露.
主要成果:
- 在L. plantarum.中成功生成和表面显示了两个新抗原 (NAG1,ETV6).
- 证明成功地将新抗原向细胞膜和细胞壁.
- 根据定方法观察到不同的生产水平和表面暴露.
- 发现细胞壁固的抗原与细胞膜固的抗原相比,表现出更高的表面暴露.
结论:
- L. plantarum可以被设计成在其表面产生和显示新抗原.
- 细胞壁是最大限度地增加新抗原表面暴露的更有效的策略.
- 这项研究提供了第一个关于乳杆菌中新抗原生产和表面显示的报告,为癌症免疫疗法开辟了新的途径.
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