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对甲状腺黄病毒的风险评估及其对蚊子病原性黄病毒复制的影响

Yumei Fu^1,2, Wan Zhao¹, Shaohui Wu^1,2

¹State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Parasites & vectors

|March 5, 2025

概括

昆虫特异性黄状病毒 (ISF),如甲虫黄状病毒 (AEFV),不构成人类健康风险. 然而,蚊子感染AEFV可能会降低它们传播危险树木病毒的能力,如寨卡病毒和登革热.

科学领域:

病毒学病毒学
医学昆虫学医学昆虫学
分子生物学分子生物学

背景情况:

蚊子可以传播具有医学意义的黄病毒.
越来越多地发现了昆虫特异性黄病毒 (ISF).
需要评估ISF对人类健康的风险和传播影响.

研究的目的:

为了研究海南菌株甲虫黄斑病毒 (Aedes flavivirus,AEFV) 在各种细胞系中的传染性.
评估AEFV对人类健康的潜在风险.
探索AEFV对Aedes albopictus病原性树病毒的载体能力的影响.

主要方法:

隔离了AEFV海南菌株的艾滋病白斑.
在Aedes,Culex和哺乳动物细胞系中使用FISH和qPCR进行感染测定.
评估AEFV对树冠病毒复制和蚊子免疫路径的影响.

主要成果:

AEFV海南菌株在遗传上与其他菌株相似.
由于细胞进入障碍,AEFV感染的是Aedes蚊子,但不是Culex quinquefasciatus.
在哺乳动物细胞中,AEFV的感染风险较低.
通过免疫路径激活,AEFV的预感染可以减少寨卡病毒和登革热病毒在Aedes albopictus中的复制.

结论:

AEFV海南菌株对人类健康没有危险.
在蚊子中,AEFV可以起到免疫诱导作用.
AEFV有可能破坏蚊子传播病原性树木病毒的传播.

关键词:

艾得斯埃吉普提病毒 (Aedes aegypti) 是一个艾迪斯白斑虫 (Aedes albopictus) 是一个白斑虫.昆克法西雅图斯 (Culex quinquefasciatus) 是一个五形状的植物.艾滋病黄病毒 (Aedes flavivirus) 是一种病毒.登革热病毒感染者特定于昆虫的黄斑病毒.哺乳动物细胞是哺乳动物细胞.寨卡病毒寨卡病毒

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