基-tRNA合成酶通过mTORC1-LAT1通路调节奶牛中的素合成
Yongding Ke1, Ximeng Du1, Binglan Chen1
1Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, China.
Animal bioscience
|March 6, 2025
概括
基-tRNA合成酶 (LARS) 通过增强素运输和激活mTORC1通路来提高乳酪蛋白的产量. 这种酶调节L型氨基酸载体1 (LAT1) 的表达和定位,这对蛋白质合成至关重要.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 乳制品科学 乳制品科学
背景情况:
- 基-tRNA合成酶 (LARS) 是哺乳动物的一个关键酶,对蛋白质合成和氨基酸平衡至关重要.
- 拉斯作为细胞内白蛋白传感器,通过Rag GTPase结合激活mTORC1通路,以调节蛋白质合成.
- 对于LARS多功能作用的精确机制,特别是在牛奶蛋白质合成中,需要进一步阐明.
研究的目的:
- 研究Leucyl-tRNA合成酶 (LARS) 在调节牛乳表皮细胞中的牛奶蛋白合成中的作用.
- 阐明LARS影响素生产和细胞过程的分子机制.
主要方法:
- 使用了稳定的牛乳腺上皮细胞系 (MAC-T) 与LARS过度表达和淘汰.
- 使用细胞计数套件-8,Click-iT EdU,西斑,实时定量PCR和免疫聚焦显微镜进行验证.
主要成果:
- 在MAC-T细胞中LARS过度表达增强了增殖和细胞内白水平,通过mTORC1-LAT1通路增加了素的产生.
- LARS积极调节L型氨基酸载体1 (LAT1) 的表达和膜局部化,由LAT1在mTORC1.1上的反机制支持.
- LARS通过其在翻译和白蛋白传感中的双重作用影响LAT1表达,而LAT1局部化仅依赖于白蛋白传感.
结论:
- 通过mTORC1通路,LARS调节LAT1表达和膜定位,受细胞内白蛋白水平的影响,从而影响素合成.
- 这些发现为提高牛奶蛋白质生产和改善乳制品质量提供了理论基础.
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