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氧化物暴露在斑马鱼胚胎中通过亡途径诱导毒性和心脏毒性

Yan Liu¹, Ruxia Pu¹, Bo Zou¹

¹School of Basic Medical Sciences and Forensic Medicine, Baotou Medical College, Baotou, China.

Journal of applied toxicology : JAT

|March 6, 2025

概括

在斑马鱼胚胎中,氧化物 (Sm2O3) 暴露导致发育问题,如胀和缩短长度. Sm2O3引发反应性氧物种和亡,突出其潜在的生物危害.

科学领域:

环境科学环境科学
毒理学毒理学毒理学
发育生物学发展生物学

背景情况:

(Sm) 和氧化 (Sm2O3),轻质稀土元素,在电子,化学和医学方面广泛应用.
越来越多的Sm2O3在环境中存在和生物积累,需要了解它对人类健康的影响.

研究的目的:

研究斑马鱼胚胎中Sm2O3暴露的毒性影响和潜在机制.
为了澄清Sm2O3在脊椎动物早期发育中的潜在生物危害.

主要方法:

在受精后24小时 (hpf) 的斑马鱼胚胎暴露于Sm2O3.
使用显微镜 (SEM,TEM,激光对焦) 评估发育异常.
通过Lyz-fish系统分析细胞损伤,阿克里丁色,蛋白质表达 (Bcl-2,Caspase-3) 和反应性氧物种 (ROS) 检测 (DCFH-DA).

主要成果:

Sm2O3引发了显著的发育异常,包括心周胀,脊柱曲和体长缩短.
Sm2O3的积累导致皮肤细胞微缩和巨细胞迁移,表明组织受损.
观察到心脏异常,如心室胀,缩和心律失常,与增加的ROS和亡有关.

结论:

暴露于Sm2O3会促进ROS的积累,并激活斑马鱼胚胎中的卡斯巴酶依赖的亡途径.
这些机制导致发育性毒性,包括心周胀和心脏功能障碍.
该研究强调了Sm2O3在脊椎动物早期发育过程中的潜在生物危害.

关键词:

在Sm2O3中,Sm2O3是什么?灭症 (apoptosis) 是一种死亡的过程.心脏有毒性心脏有毒性稀土是一种稀土.斑马鱼是一种斑马鱼.

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