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核糖体蛋白S25促进细胞循环进入一个富有成效的BK多聚马病毒感染

J M Needham¹, T M Greco², I M Cristea²

¹Department of Microbiology, The University of Alabama at Birmingham, Birmingham, AL 35205, USA.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

|March 6, 2025

概括

乙基多重瘤病毒 (BKPyV) 需要核糖体蛋白S25 (eS25) 才能有效地产生病毒,而不依赖于翻译启动. 这项研究揭示了ES25的存在.

科学领域:

病毒学病毒学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

由于编码能力有限,病毒经常使用非正典的翻译启动.
核糖体蛋白S25 (eS25) 对于像IRES这样的替代翻译启动机制至关重要.
eS25对于上限依赖的翻译来说并不必不可少,这使得它的敲击成为研究病毒依赖非正典途径的工具.

研究的目的:

为了调查BK多瘤病毒 (BKPyV) 是否利用内部核糖体进入部位 (IRES) 来进行翻译.
确定核糖体蛋白S25 (eS25) 在BKPyV复制中的作用.
探索eS25对病毒感染期间宿主细胞周期进展的影响.

主要方法:

BKPyV病毒产量通过敲击来评估eS25的存在和缺乏.
细胞循环分析是在初级细胞上进行的,使用eS25敲击.
研究了与基因表达相关的病毒生产时间.

主要成果:

在基因表达之前,BKPyV强大的病毒产生依赖于eS25,而不是IRES介导的翻译.
在初级细胞中,eS25 knockdown导致细胞周期在G0/G1和G2/M阶段停止.
BKPyV感染的时间受宿主细胞初始细胞周期状态的影响.

结论:

BKPyV复制依赖于eS25在其生命周期的早期阶段,与依赖IRES的翻译不同.
eS25在调节宿主细胞周期进展方面发挥着重要作用.
宿主细胞周期状态是影响BKPyV感染动态的关键因素.

关键词:

在BK多重病毒中,BK多重病毒.在RPS25中使用RPS25.细胞循环中的细胞循环.在 eS25 里面,你会看到 eS25 .开始翻译开始翻译

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