病毒图:用于病毒整合分析和可视化的Web服务器
Erqiang Hu1,2,3, Jianhong An1,2,3, Adam J Gersten1,4
1Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, United States.
Frontiers in oncology
|March 6, 2025
概括
病毒DNA整合到人类基因组中,通过引起基因组不稳定,驱动癌症. 我们开发了virusPlot,这是一个用于分析和可视化这些病毒集成站点的网络工具,为研究人员简化了复杂的数据.
科学领域:
- 基因组学和生物信息学
- 瘤学和病毒学
背景情况:
- 病毒DNA融入人类基因组是癌症发展的关键因素,导致基因组不稳定性和改变基因表达.
- 目前用于分析病毒整合的计算工具有限,特别是用于可视化和解释的基于Web的应用程序.
研究的目的:
- 开发和介绍virusPlot,一个新的Web服务器,旨在分析和可视化病毒DNA集成到宿主基因组.
- 为研究人员提供一个直观和用户友好的工具,以简化复杂的病毒整合模式的解释.
主要方法:
- 开发了virusPlot作为一个使用shinyapp.pp.的交互式网络平台.
- 实现了用于自动检索和注释病毒基因组序列的模块.
- 综合可视化病毒和宿主基因组集成站点,读数,并使用费舍尔精确测试分析集成热点的分析.
主要成果:
- VirusPlot成功地处理和可视化病毒整合数据,将病毒和宿主基因组整合站点连接起来.
- 该工具有助于识别整合模式和热点.
- 提供交互式图形表示,以便更轻松地解释集成数据.
结论:
- VirusPlot解决了在病毒整合分析中需要专门的计算工具的需求.
- 网络服务器为研究人员提供了一种高效和用户友好的解决方案,用于研究癌症发病的病毒宿主基因组相互作用.
- 增强对病毒整合机制及其在癌症中的作用的理解.
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