聚β-氨基乙烯的酶合成聚乙烯配合聚合物具有高强度的消除阴性细菌
Peng Dong1, Wei Xiong2, Jin Feng1
1Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, P. R. China.
Macromolecular rapid communications
|March 6, 2025
概括
这项研究引入了一种由BocOP合成的新型聚β-氨基,通过破坏其膜来证明它对Gram负细菌具有有效的杀菌特性. 由此产生的聚合物显示出低血解和细胞毒性.
科学领域:
- 聚合物化学 聚合物化学
- 生物材料科学 生物材料科学
- 抗菌剂 抗菌剂 抗菌剂
背景情况:
- 开发新的抗菌剂对于对抗细菌感染至关重要.
- 聚β氨基 (PAE) 作为具有可调节性质的生物相容材料具有潜力.
- 酶聚合为聚合物合成提供了一条绿色和高效的途径.
研究的目的:
- 为了合成一个功能性乳,2-甲基-2-乙烯-7-oxo-1,4-oxazepane-4-carboxylate (BocOP).
- 通过使用不动化的脂酶B.研究BocOP的同聚合和共聚合动力学.
- 评估由此产生的PAE共聚物的抗菌活性和生物相容性.
主要方法:
- 通过氧子介导的 Baeyer-Villiger 氧化合成 BocOP.
- 由被动化的Candida antarctia lipase B (Novozym 435) 催化的BocOP的酶同聚合.
- 使用 methoxypolyethylene glycols (mPEG) 作为启动剂的 BocOP 与 ε-caprolactone (CL) 的酶共聚化.
- 降低PAE共聚物的保护,以揭示二次氨基基组.
- 对抗格拉姆阴性细菌,血液溶解和细胞毒性的杀菌作用的评估.
主要成果:
- 通过使用Novozym 435. BocOP成功合成并聚合成PAE.
- 随机合成了BocOP和CL的PAE共聚物.
- 无保护的PAE共聚物对格拉姆阴性细菌表现出优越的杀菌活性.
- 该机制涉及细菌外部和内部膜的破坏.
- 在PAE共聚物中观察到低血解和细胞毒性.
结论:
- 功能PAE共聚合物可以通过BocOP的酶聚合来有效合成.
- 开发的PAE共聚物显示出强大且有针对性的抗微生物活性,对抗格兰氏阴性细菌.
- 这些PAE共聚合物代表了抗微生物应用的有希望的候选者,具有有利的安全性.
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