茶外细胞膀衍生的微RNAs通过重编程巨细胞,有助于缓解肠道炎症
Tianyu Luo1, Linhai Hou1, Yaqi Cao1
1School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Journal of agricultural and food chemistry
|March 6, 2025
概括
茶叶衍生的细胞外囊泡 (TEVs) 重新编程巨细胞以减少炎症性肠病 (IBD) 中的炎症. 这些天然囊泡含有微RNA,通过调节基因表达和巨细胞两极分化来缓解大肠炎.
科学领域:
- 生物医学科学 生物医学科学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 传统的炎症性肠病 (IBD) 治疗由于副作用而受到限制.
- 来自植物性饮食的细胞外囊泡显示出缓解疾病的潜力.
研究的目的:
- 研究茶叶衍生的细胞外囊泡 (TEVs) 在炎症性肠病中的治疗潜力.
- 阐明TEVs调节巨细胞极化和炎症的机制.
主要方法:
- 在体外和体内研究使用DSS诱导的大肠炎模型.
- 小RNA测序用于在TEV中识别微RNA.
- 巨细胞吸收测定和基因表达分析.
主要成果:
- TEVs有效地将巨细胞从一种促炎 (M1) 状态转移到一种抗炎 (M2) 状态.
- TEVs在体内改善了结肠炎,治疗效果与它们的RNA含量有关.
- 在TEV中,特定的微RNA (osa-miR166d-5p, gma-miR396a-3p) 被确定为抗炎作用的关键媒介.
结论:
- TEVs代表了炎症性肠病的有前途的天然治疗方法.
- TEVs通过通过特定的microRNAs重新编程巨细胞极化来产生抗炎作用.
- 鉴定的microRNAs针对关键的炎症途径,提供了新的治疗点.
更多相关视频
07:34Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
Published on: December 16, 2021
2.4K
06:57Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
Published on: September 18, 2016
9.0K
相关概念视频
Renewal of Intestinal Stem Cells
2.5K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.5K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.0K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.0K
MicroRNAs
21.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
