对SARS-CoV-2变种和季节性冠状病毒在环境中持久性的比较分析
Geun Woo Park1, Boris Reija1,2, Azaibi Tamin3
1Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Applied and environmental microbiology
|March 6, 2025
概括
像OC43和牛冠状病毒 (BoCoV) 这样的季节性冠状病毒在表面上并不完全模仿SARS-CoV-2变种. 表面类型显著影响病毒的持久性,警告不要将其用作环境研究中的直接替代品.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 传染病研究传染病研究.
背景情况:
- 进行严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 持久性研究通常需要生物安全级别3 (BSL-3) 实验室.
- 人类季节性冠状病毒 (229E,NL63,OC43) 和牛冠状病毒 (BoCoV) 是BSL-2病原体,可能作为替代品.
研究的目的:
- 为了比较BSL-2人类冠状病毒 (229E,NL63,OC43) 和牛冠状病毒 (BoCoV) 与SARS-CoV-2变种 (WA-1,Delta,Omicron) 的环境持久性.
- 评估这些BSL-2病毒在环境持久性研究中作为SARS-CoV-2的替代品的适用性.
主要方法:
- 在室温下,OC43和BoCoV的持久性在不钢,塑料,乙烯基和触摸屏玻璃券上进行了测试.
- 在不钢和触摸屏玻璃券上评估了SARS-CoV-2变种的生存能力.
- 随着时间的推移,使用CT值监测病毒RNA标位.
主要成果:
- OC43和BoCoV表现出不同的持久性模式,BoCoV在大多数表面上持久时间比OC43更长.
- SARS-CoV-2 变种在不同的表面上表现出不同的持久性,在触摸屏玻璃上具有更长的活力.
- 无论是BoCoV还是OC43,在所有测试的表面上都没有完全复制SARS-CoV-2变种的环境持久性特征.
结论:
- 与SARS-CoV-2变种相比,牛类冠状病毒和OC43的环境持久性不同.
- 表面类型显著影响病毒的持久性,突出使用代用病毒时的局限性.
- 在环境表面持久性研究中,建议在使用OC43,NL63,229E或BoCoV作为SARS-CoV-2的替代品时谨慎使用.
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