全基因组转录学揭示了新生儿高氧反应的不同心房与心室反应
E David Cohen1, Min Yee2, Kyle Roethlin2
1Department of Pediatrics, Division of Cardiology, School of Medicine and Dentistry, The University of Rochester, Rochester, New York, United States.
概括
在早产婴儿 (过氧) 中,早期暴露氧气会导致晚期心力衰竭. 这项研究表明,高氧症独特地损害了左心房,导致心脏问题,并建议早产儿进行室内特定的治疗.
科学领域:
- 心脏病学 心脏病学
- 新生儿生理学 新生儿生理学
- 分子生物学分子生物学
背景情况:
- 过早出生的婴儿往往需要补充氧气 (过氧),这与晚年心力衰竭有关.
- 在动物模型中的新生儿高氧症在早产成年人中观察到的人类心脏病.
- 过氧不同影响新生儿心室,影响心肌细胞的增殖和存活.
研究的目的:
- 为了研究新生儿心脏对高氧化的室内特异性分子和细胞反应.
- 识别生命早期高氧引起的心脏功能障碍背后的机制.
- 为了比较左心室与左心室在过氧暴露后的转录变化.
主要方法:
- 进行了全基因组转录 (RNA测序) 在新生小鼠左心室和心室暴露于高氧化.
- 分析包括基因表达概况,DNA损伤评估和与压力有关的途径的评估.
- 关键的分子标记物如p53,ATM激酶,线粒体压力指标和SOD3被量化.
主要成果:
- 左心室对高氧反应的转录反应明显大于左心室 (4,285对1,743个受影响的基因).
- 与心室相比,高氧引起的DNA损伤,ATM激酶酸化,线粒体应激和心房中的亡更大.
- 心室显示DNA修复和压力颗粒基因的表达增加,而心室显示细胞外矩阵和SOD3表达减少.
结论:
- 新生儿左心室比左心室更容易受到过氧的影响,具有不同的分子反应.
- 大心室细胞外矩阵损失和减少的SOD3可能会导致腔室扩大和腹功能障碍.
- 室内特定的治疗策略可能是必要的,以预防或治疗早产婴儿暴露于高氧化的心力衰竭.
更多相关视频
11:26Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer
Published on: February 13, 2019
8.6K
10:03Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
Published on: May 12, 2023
2.3K
相关概念视频
01:14Fetal Circulation
Fetal circulation is a unique system that facilitates the exchange of gases, nutrients, and waste products between the developing fetus and the mother. This intricate process takes place through a special organ called the placenta.
Two umbilical arteries transport blood from the fetus to the placenta. At the placenta, the blood absorbs oxygen and nutrients while simultaneously eliminating waste products. This oxygen-enriched and nutrient-rich blood then returns to the fetus through one...
Two umbilical arteries transport blood from the fetus to the placenta. At the placenta, the blood absorbs oxygen and nutrients while simultaneously eliminating waste products. This oxygen-enriched and nutrient-rich blood then returns to the fetus through one...
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...