流程优化,以改善海上孤立的Bacillus haynesii和Saccharomyces cerevisiae生物乙醇生产的提丁酶生产
Vishnupriya Govindaraj1, Dinesh Kumar Anandan1, Se-Kwon Kim2
1Department of Chemical Engineering, National Institute of Technology, Surathkal, Mangalore, 575025, Karnataka, India.
概括
这项研究优化了来自Bacillus haynesii的基因酶生产,以获得可持续的生物乙醇. 优化的条件产生了显著的酶活性,为有效地将基废物转化为有价值的生物乙醇燃料铺平了道路.
科学领域:
- 生物技术是生物技术.
- 生物化学 生物化学
- 环境科学 环境科学
背景情况:
- 基是第二大生物资源,为生物炼油厂提供了纤维素的可持续替代品.
- 海鲜加工会产生大量的贝废物,这为利用基基方法的价值化提供了机会.
- 开发有效的基降解方法对于释放其在生物乙醇生产和废物管理方面的潜力至关重要.
研究的目的:
- 为了优化从海洋分离的Bacillus haynesii中提丁酶的生产,以提高生物乙醇的产生.
- 确定最佳的过程参数和介质组件,以最大限度地提高基因酶产量和活性.
- 评估Bacillus haynesii衍生的素寡糖的效率,作为Saccharomyces cerevisiae生物乙醇生产的基质.
主要方法:
- 每次使用一个因素 (OFAT) 优化了诸如注射器年龄,大小,温度,pH值和填充量等参数.
- 普莱克特-伯曼设计 (PBD) 选了媒体组件,随后是塔古奇正角数组优化.
- 乙醇产量使用二酸盐氧化方法量化.
主要成果:
- 结合体胆被确定为Bacillus haynesii生长的最佳碳来源.
- 优化的介质组成 (酵母提取物,甘油,合基,MnCl2·4H2O,MgSO4·7H2O) 产生了高酶活性 (6.85 U/mL) 和特异性活性 (28.87 U/mg).
- 生物乙醇度为2.48%v/v,从33.18g/L的基寡糖化物中获得.
结论:
- 菌根是基酶生产的有希望的来源,对于基基生物炼油厂至关重要.
- 优化的发酵条件显著提高了酸酶的产量和活性.
- 来自Bacillus haynesii的基寡糖是可持续生物乙醇生产的可行基质,有助于废物利用.
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