合成遗传元素能够快速描述Cupriavidus necator H16中的无机碳吸收系统
Akira K Nakamura1,2, Emily M Fulk3, Christopher W Johnson3
1Department of Molecular, Cellular & Developmental Biology, Yale University, New Haven, Connecticut 06520, United States.
ACS synthetic biology
|March 6, 2025
概括
这项研究为Cupriavidus necator H16引入了一种新的基因组工程技术,使得高效的合成遗传元素集成成为可能. 这促进了碳负生物制造,并提高了细胞内二碳酸盐度.
科学领域:
- 微生物学 微生物学
- 合成生物学 合成生物学
- 生物技术是生物技术.
背景情况:
- 库普里亚维杜斯 (Cupriavidus necator H16) 是一种具有碳负生物制造潜力的可选化基类植物.
- 有限的基因工程工具阻碍了C. necator在研究和工业中的应用.
研究的目的:
- 为C. necator.开发一种高效的基因组工程技术.
- 建立一种使用合成遗传元素 (SGE) 调节基因表达的方法.
- 为了增强C. necator.中的无机碳吸收.
主要方法:
- 开发了一种基因组工程技术,用于在C. necator中调动,集成和表达SGE.
- 测试了四个可诱导的促进器,以优化可调节基因表达的遗传着陆台.
- 使用SGE系统设计,调动和表达了八种异构的无机碳吸收途径.
主要成果:
- 成功建立了C. necator.的基因组工程技术.
- 优化了一个基因着陆台,用于可调节的基因表达.
- 在C. necator中证明了细胞内二碳酸盐度的上调,具有工程碳吸收途径.
结论:
- 该SGE策略有助于快速整合和调节表达C. necator.中异质路径.
- 这项工作提高了细胞内二碳酸盐度,这对于生物制造应用至关重要.
- 开发的技术扩大了C.necator对基础和应用研究的实用性.
更多相关视频
07:32Monitoring Pedogenic Inorganic Carbon Accumulation Due to Weathering of Amended Silicate Minerals in Agricultural Soils.
Published on: June 4, 2021
5.0K
08:21Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
Published on: August 15, 2017
12.8K
相关概念视频
C4 Pathway and CAM
45.1K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.1K
The Calvin Benson Cycle
4.4K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.4K
Key Elements for Plant Nutrition
18.6K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
18.6K
Water and Mineral Acquisition
28.9K
Specialized tissues in plant roots have evolved to capture water, minerals, and some ions from the soil. Roots exhibit a variety of branching patterns that facilitate this process. The outermost root cells have specialized structures called root hairs that increase the root surface, thus increasing soil contact. Water can passively cross into roots, as the concentration of water in the soil is higher than that of the root tissue. Minerals, in contrast, are actively transported into root cells.
28.9K
Overview of Transposition and Recombination
15.2K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.2K
Transgenic Plants
7.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.1K
