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在人类鼻上表皮的多细胞中,可变DPP4表达是MERS-CoV热流的决定因素

Tim I Breugem¹, Samra Riesebosch¹, Jingshu Zhang¹

¹Viroscience Department, Erasmus University Medical Center, Rotterdam 3015 GD, The Netherlands.

Proceedings of the National Academy of Sciences of the United States of America

|March 6, 2025

概括

中东呼吸综合征冠状病毒 (MERS-CoV) 针对上呼吸道的多细胞,尽管受体表达有限. 受体存在的个体变异解释了MERS-CoV.

科学领域:

病毒学病毒学
呼吸系统医学呼吸系统医学
细胞生物学细胞生物学

背景情况:

病毒的传染性与热带性有关,许多呼吸道病毒向上呼吸道多细胞.
中东呼吸综合征冠状病毒 (MERS-CoV) 由于上呼吸道受体 (二基酸酶4 - DPP4) 的有限表达,通常表现为人对人传播不良.
MERS-CoV流行病学包括超级传播事件,这表明传输效率的个体变化.

研究的目的:

使用人类呼吸道器官来研究MERS-CoV的呼吸道热带性.
确定MERS-CoV在上下呼吸道中的细胞点.
探索受体表达变异性在MERS-CoV传播中的作用.

主要方法:

使用了分化良好的人类肺部和鼻腔气道器官衍生物培养物.
采用单细胞信使RNA测序,免疫光和免疫组织化学.
在差异化呼吸道培养中分析了MERS-CoV复制量和细胞热带性.

主要成果:

在肺部和鼻腔气道有机体培养物中,MERS-CoV复制到高位.
该病毒首选向多细胞,导致毛覆盖的丧失.
细胞热带性取决于器官分化,在复制效率和DPP4表达方面,有显著的捐赠者对捐赠者的变化.

结论:

在人体上空和下空气道中,MERS-CoV表现出多细胞的热带性.
在上呼吸道内,DPP4表达的个体变化会影响MERS-CoV的热带性和传播.
这项研究为在MERS-CoV爆发中观察到的不可预测的传播模式提供了潜在的解释.

关键词:

DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 这就是MERS-CoV.冠状病毒冠状病毒的新冠病毒.鼻子鼻子鼻子鼻子有机生物有机物

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