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突尼斯多药耐药Shigella sonnei疫情的分子流行病学 (2022-2023) 使用全基因组测序

Fahmi Smaoui^1,2, Boutheina Ksibi^1,2, Senda Mezghani^1,2

¹Research Laboratory Microorganisms and Human Disease 'MPH LR03SP03', Laboratory of Microbiology, Habib Bourguiba University Hospital, Sfax, Tunisia.

Microbial genomics

|March 6, 2025

概括

突尼斯的一次多抗药性Shigella sonnei疫情与HC10-20662基因型有关. 这种在欧洲发现的基因型传播到突尼斯,突出了全球对抗菌素耐药性的基因组监测的需要.

科学领域:

微生物学微生物学
基因组学就是基因组学.
公共卫生公共卫生

背景情况:

全球多药耐药 (MDR) *Shigella sonnei*的全球流行是一个日益严重的公共卫生问题.
2022年,在突尼斯发现了一个MDR *S. sonnei*疫情.

研究的目的:

调查突尼斯疫情期间*S. sonnei*分离的遗传特征.
确定克隆关系,抗菌素耐药性基因和疫情菌株的国际联系.

主要方法:

来自突尼斯南部的24种*S. sonnei*菌株的全基因组测序 (2022年7月 - 2023年11月).
生物信息分析用于物种识别,序列类型,遗传学分析和抗菌素耐药性决定因素的识别.
植物动力学和植物地理学分析,以追踪疫情的时空传播.

主要成果:

在24个分离物中,有23个属于3.6.3亚类中的HC10-20662基因型.
所有的分离物都携带了 *blaCTX-M-15*, *dfrA1*, *qnrS1* 和 *gyrA* D87G 突变. 抗性基因 *sul2*, *tet(A) * 和 *mph(A) * 的流行率很高.
人类地理分析表明,疫情的基因型可能起源于欧洲,然后蔓延到突尼斯.

结论:

这项研究报告了突尼斯首次MDR * S. sonnei*疫情,由HC10-20662基因型驱动.
HC10-20662基因型与突尼斯和欧洲的多国疫情有关.
持续的国家和国际基因组监测对于监测MDR * S. sonnei* 演变和传播至关重要.

关键词:

希格拉·索内伊 (Shigella sonnei) 是一个小鸟.突尼斯突尼斯突尼斯突尼斯多种药物耐药性多种药物耐药性进行全基因组测序.

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