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Updated: May 24, 2025

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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揭开异能潜力:洞察细胞自由的微RNA与蛋白质相互作用

Vishal Kumar Sahu1, Subhayan Sur1, Sanjana Agarwal1

  • 1Cancer and Translational Research Centre, Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Tathawade, Pune 411033, India.

Biophysical chemistry
|March 6, 2025
PubMed
概括

这项研究引入了一种计算方法来分析细胞外微RNA-蛋白相互作用,识别关键模式和潜在的疾病生物标志物. 这些发现为疾病机制和治疗点提供了新的见解.

关键词:
没有细胞的miRNA.在电话会议上.这是一个miRNA-蛋白质数据库.这就是miRNA-蛋白质对接.的miRNA-蛋白相互作用.

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MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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