发芽酵母Ykt6的双化调节了细胞壁的完整性和自
Masaki Tateishi1, Kota Goto1, Eiji Hishinuma2
1Department of Molecular and Cellular Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
The Journal of biological chemistry
|March 6, 2025
概括
酵母蛋白 Ecm9 能够保持 Ykt6 的双化,Ykt6 是 SNARE 蛋白质,对于膜贩运和自是必不可少的. 这种修改对Ykt6至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 蛋白质修饰是一种蛋白质修饰.
背景情况:
- Ykt6是一种保存的SNARE蛋白质,对膜贩运至关重要,包括Golgi内部运输和自融合.
- 哺乳动物Ykt6在C端经历了双重前化 (法内西尔和格拉尼尔格拉尼尔),这种修饰的进化保存不确定.
研究的目的:
- 为了研究Ykt6在真核生物中的双重化过程的进化保存.
- 为了识别发芽酵母中第二个前化步骤的酶.
- 为了确定Ykt6双化的功能意义.
主要方法:
- 利用结构预测来识别酵母体3型 (GGT3) 基拉尼尔基拉尼尔转移酶的潜在子单元.
- 研究了Ecm9和Bet2.2.之间的蛋白质复合体形成.
- 采用MALDI-TOF/TOF质谱测量来确认Ykt6在野生类型和突变酵母菌株中的prenylation状态.
- 评估了细胞壁完整性,Golgi mannosyltransferase局部化,以及缺乏Ecm9.9的酵母中的自活性.
主要成果:
- 鉴定出Ecm9是酵母GGT3的功能性α子单元,该子单元负责基基基化单基化Ykt6.6.
- 已证实,芽酵母Ykt6确实被双倍化,这证实了这种修饰的进化保存.
- 证明Ecm9的损失导致细胞壁脆弱,影响Ykt6膜局部化,并减少自活动.
结论:
- Ykt6的双化是一种进化保存的特征,对于其适当的膜局部化至关重要.
- 通过ECM9介导的GGT3活性对于Ykt6在膜贩运和自中的功能至关重要.
- 干扰Ykt6前化会影响细胞过程超越膜融合,包括细胞壁完整性.
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