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人类精子的组成sncRNAome:一个跨国小型RNA分析

Poonam Mehta^1,2, Rajender Singh^3,4

¹CSIR-Central Drug Research Institute, Lucknow, India.

Reproductive biology and endocrinology : RB&E

|March 6, 2025

概括

人类精子小非编码RNAs (sncRNAs) 显示了一个核心组件具有微小的变化和一个外围组件具有显著的变化跨个体和种群. 了解这些变异对于生育研究至关重要.

科学领域:

生殖生物学生殖生物学
遗传学遗传学是一个
分子生物学分子生物学

背景情况:

精子传递的小型非编码RNA (sncRNAs) 与生育和跨代遗传有关.
人类精子sncRNA含量 (sncRNAome) 的个体间和人群间变异在很大程度上仍然没有特征.

研究的目的:

为了确定人类精子sncRNAome的个体间和种群间的变化.
在不同的人群中识别精子sncRNA配置文件的相似性和差异.

主要方法:

在54个印度normzoospermic捐赠者身上进行了sncRNA测序.
分析了来自中国,美国和西班牙 normozoospermic 样本的公开可用的 sncRNA 测序数据.
在种群内和跨种群的sncRNA数据进行比较,以确定变异.

主要成果:

主要的sncRNA部分包括重复小RNAs (rsRNAs),YRNAs (YsRNAs) 和tRNA衍生的小RNAs (tsRNAs).
只有微RNAs (miRNAs) 和piwiRNAs (piRNAs) 的一个小子集在不同种群中一致,piRNAs主要来自15号染色体.
tRNA-Gly-GCC是主要的tsRNA贡献者,Y4sRNA是最丰富的YsRNA,其他贡献者的种群特异性变化.

结论:

人类精子sncRNAome表现出一个保存的"核心成分"与最小的变化和一个可变的"外围成分".
在个人和种群中存在精子sncRNA配置文件的显著变化.
描述正常的人类精子sncRNAome对于区分生物变异和随机样本差异至关重要.

关键词:

人类精子RNAs 精子RNAs是一种精子RNA.精子中的小RNAs.精子 sncRNAome 的精子跨代遗传是一种跨代遗传.

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