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克服冰:尖端材料和先进的策略,有效的生物样本的冷保存

Miaorong Huang^1,2, Minhua Hu³, Gengyuan Cai^1,2

¹State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.

Journal of nanobiotechnology

|March 6, 2025

概括

冷保存面临着冰形成和有毒冷保护剂 (CPA) 的挑战. 新材料,生物化学调节和纳米技术等先进技术是改善生物样品冷保存的关键.

科学领域:

生物医学工程生物医学工程
低温生物学低温生物学
材料科学材料科学材料科学

背景情况:

冷保存对于生物医学应用和生殖质的保存至关重要,旨在保持生物材料的完整性.
目前的限制包括冰形成损伤和常规冷保护剂 (CPA) 的细胞毒性.
需要采用整体方法来克服冷保存中的这些挑战.

研究的目的:

审查冷保存中的基本损伤机制.
评估目前的冷保护剂及其局限性.
探索新的材料和技术,以提高冷保存结果.

主要方法:

总结了冷保存损伤机制和CPA限制.
评估了与冰相互作用的材料 (糖,聚合物,AFP,冰核子,水凝).
讨论了生物化学调节,纳米技术,细胞封装,冷网和同位体冷.

主要成果:

确定了冰形成和CPA细胞毒性作为主要的冷保存障碍.
强调了各种材料和先进技术在减轻损坏方面的潜力.
强调需要多学科的方法.

结论:

冷保存的进步需要协同战略,将冷生物学与新兴技术结合起来.
未来的方向包括整合合成生物学,纳米技术,微流体学和3D生物打印以实现高效的冷保存.
跨学科的进步对于克服当前冷保存的局限性至关重要.

关键词:

在3D生物打印中使用3D生物打印抗氧化剂是一种抗氧化剂.结冷保存是一种冷保存.水凝是一种水凝.微流体学微流体学纳米技术纳米技术

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Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...