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黑色素细胞是否在控制表皮细菌殖民和皮肤微生物组方面发挥作用?
Omera Bi1,2, David Caballero-Lima2, Stephen Sikkink1
1Centre for Skin Sciences, Faculty of Life Science, University of Bradford, Bradford, UK.
3D皮肤模型中的功能性黑色素细胞显著减少了细菌殖民,并调节了免疫基因表达,突出了它们在皮肤免疫中超越色素的作用.
科学领域:
- 皮肤病学 皮肤病学
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- 黑色素细胞产生黑色素以保护紫外线,并可能在天生的皮肤免疫力中发挥作用.
- 皮肤微生物组涉及到开始性和致病性细菌之间的复杂平衡.
研究的目的:
- 为了研究黑色素细胞对表皮细菌生长的影响.
- 为了确定黑色素细胞是否调节免疫基因表达在3D皮肤模型中.
主要方法:
- 开发了一种3细胞全厚的人类皮肤,相当于黑色细胞,纤维细胞和角质细胞.
- 对比细菌殖民和免疫基因表达在有色素与无色素的皮肤等价物.
- 利用免疫组织化学和阿尔法黑色素细胞刺激激素 (α-MSH) 刺激.
主要成果:
- 功能性黑色素细胞显著降低了细菌的殖民化 (白杆菌 (Staphylococcus epidermidis),状细菌 (Corynebacterium striatum),状细菌 (Cutibacterium acnes).
- 黑色素细胞在微生物暴露时调节免疫反应基因 (S100A9,DEFB4A,IL-4R) 的表达.
- 在有色素和无色素皮肤对应物之间观察到免疫基因表达的差异.
结论:
- 功能性黑色素细胞增强了皮肤对细菌病原体的天生的免疫力.
- 3D皮肤等效模型对于研究皮肤色素,免疫力和微生物群相互作用是有价值的.
- 这个模型为皮肤疾病,黑色素瘤,紫外线损伤和微生物组研究提供了新的研究途径.
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