lncRNA1471通过与ASR转录因子结合来调解番茄成熟的开始
Lingling Zhang1,2, Guoning Zhu1, Liqun Ma1
1College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
The Plant journal : for cell and molecular biology
|March 7, 2025
概括
长非编码RNA 1471 (lncRNA1471) 负面调节番茄果实成熟的过程. 它的去除加速了成熟,增加了乙烯,甘和β-胡卜素,同时降低了度.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因规则 基因规则
- 发展水果发展水果发展
背景情况:
- 果实成熟是一个由荷尔蒙,转录因子和表观遗传学调节的复杂过程.
- 长非编码RNAs (lncRNAs) 在水果成熟中的作用在很大程度上尚未被探索.
研究的目的:
- 为了研究红果成熟过程中 lncRNAs 的功能.
- 为了确定参与调节成熟开始的特定 lncRNA.
主要方法:
- 基因编辑 (大片段删除) 创建一个lncRNA1471淘汰突变.
- 果实发育和成熟的表型分析.
- 关键的成熟相关基因的基因表达分析 (RT-qPCR).
- 生物化学测定乙烯,甘,β-胡卜素和水果坚硬度.
- 通过RNA净化与质谱学 (ChIRP-MS) 和体外蛋白质下拉试验相结合的染色质分离,以确定相互作用的蛋白质.
主要成果:
- 淘汰lncRNA1471加速了番茄果实成熟的开始和进展.
- 突变水果显示乙烯,甘和β-胡卜素水平增加,叶绿素降解速度更快,度降低.
- 胡卜素生物合成,乙烯生物合成和细胞壁代谢中的关键基因的表达显著改变.
- 发现 lncRNA1471 与绝缘应激成熟蛋白 (ASR) 转录因子相互作用.
结论:
- lncRNA1471作为番茄果实成熟开始的负调节剂.
- 这种lncRNA通过直接调节参与乙烯生物合成,胡卜素积累和细胞壁修饰的基因来影响成熟.
- 与ASR的相互作用表明,一种新的调节途径涉及果实成熟过程中的lncRNAs.
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