果中的EIL (乙烯不敏感3样) 转录因子会影响依赖乙烯和冷反应的水果成熟
Jiao Feng1,2, Mindy Y Wang1, Xiuyin Chen1
1The New Zealand Institute for Plant and Food Research Limited (PFR), Mount Albert Research Centre, Private Bag 92169, Auckland, 1142, New Zealand.
果的成熟过程涉及EIN3/EIN3类 (EIL) 转录因子调节乙烯信号. 操纵MdEIL基因会影响成熟,软化和香味,而EIL直接激活关键的成熟基因并对寒冷做出反应.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 果实科学 果实科学
背景情况:
- 乙烯信号对于水果成熟至关重要.
- EIN3/EIN3类 (EIL) 转录因子是乙烯信号通路中的关键调节者.
- 了解EIL在水果发育中的作用对于提高水果质量至关重要.
研究的目的:
- 研究MdEIL转录因子在果 (Malus × domestica) 果实成熟中的作用.
- 确定以乙烯为依赖和冷反应过程中MdEILs的直接目标和监管机制.
- 评估操纵MdEIL表达对水果成熟特征的影响.
主要方法:
- 转基因果线的产生与改变的MdEIL1表达 (共抑制和过度表达).
- 对水果成熟参数的分析,包括乙烯生产,软化和芳香挥发物.
- 对乙烯生物合成,细胞壁修饰,芳香生物合成和冷反应基因的基因表达分析.
- 双露西法酶记者测定和电泳性移动性转移测定以确定促进体活性和转录因子结合.
主要成果:
- 同时抑制MdEIL1-4导致乙烯的产量减少,软化和香味,而过度表达增加了成熟.
- 关键的与成熟相关的基因 (MdXTH1,MdβGAL,MdAFS1,MdoOMT1) 在共同抑制的基因系下调.
- MdEILs直接激活MdAFS1,MdXTH1和MdβGAL的促进体,MdAFS1促进体中识别了结合位点.
- 同抑制的线条显示出受寒感应的反应受损,而对寒感应的基因仍然被抑制.
结论:
- MdEIL转录因子在调节多种果成熟属性的过程中起着重要作用.
- MdEILs通过激活参与细胞壁修饰和芳香生物合成的乙烯依赖基因来起作用.
- 此外,MdEILs也对果的寒冷反应途径有所贡献,独立于乙烯信号.
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