试点规模的酸性发酵 Reground 意大利面副产品的 Polyhydroxyalkanoate 生产与混合微生物培养
Gaia Salvatori1, Angela Marchetti1, Anna Maria Russo1
1Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, Rome 00185, Italy.
概括
这项研究探讨了从意大利面副产品发酵中生产的多基酸盐 (PHAs). 不同的发酵条件产生了聚合物和聚合物,证明了废物流中的高效生物聚合物合成.
科学领域:
- 生物技术和生物化学工程 生物技术和生物化学工程
- 聚合物科学 聚合物科学
- 废弃物价值化 废弃物价值化 废弃物价值化
背景情况:
- 聚酸酸 (PHAs) 是具有多种应用的可生物降解聚合物.
- 利用食品工业的副产品,如重新面食 (RP) 用于PHA生产,提供了一个可持续的方法.
- 酸性发酵 (AF) 可以产生适合PHA合成的有机酸混合物.
研究的目的:
- 为了研究PHA生产,使用从重新面食副产品的酸性发酵中获得的有机酸混合物.
- 评估AF期间不同pH值控制策略对测序批量反应堆 (SBR) 后续PHA生产的影响.
- 描述产生的PHA,包括它们的组成和分子量.
主要方法:
- 试验规模的酸性发酵重新面 (RP) 具有和没有pH控制 (pH5.90) 产生发酵混合物 (RP发酵1和RP发酵2).
- 实验室规模的测序批量反应堆 (SBR) 运行,使用发酵混合物在短水力保留时间 (HRT) 和污泥保留时间 (SRT) 中,具有不同的有机载荷率 (OLR).
- 微生物分析和量化PHA储存产量 (COD_PHA/COD_ACIDS) 以确认微生物选择和PHA积累.
主要成果:
- 在SBR中确定了高选择性微生物压力,导致显著的PHA积累 (最大储存产量为0.68±0.04COD_PHA/COD_ACIDS).
- 经RP发酵的1产生了聚基酸/基酸) kopo 聚合物.
- 经RP发酵的2产生了一种多 (基酸/基酸/基酸) 聚合物.
- 在OLR为2.12 gCOD_ACIDS/Ld时,共聚合物和特聚合物具有相似的分子量 (分别为339 kDa和389 kDa).
结论:
- 这项研究成功地证明了使用两阶段发酵过程从重新研磨的意大利面副产品中生产PHA.
- 在最初的AF步骤中,不同的pH控制策略影响了产生的PHAs的组成.
- 在选择性条件下,SBR系统有效地积累了PHAs,突出显示了废物回收成有价值的生物聚合物的潜力.
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