微生物途径为具有成本效益的低碳可再生色素
Nawa Raj Baral1,2, Deepanwita Banerjee1,2, Thomas Eng1,2
1Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
概括
伪虫 (Pseudomonas putida) 是生产印地吉丁的顶级微生物宿主,这是一个多功能生物基分子. 优化其生产途径可以显著降低成本,并减少印地基因制造对环境的影响.
科学领域:
- 生物技术和代谢工程 生物技术和代谢工程
- 可持续的化学可持续的化学
- 工业微生物学 工业微生物学
背景情况:
- 印基因是一种有价值的生物基分子,在染料,电子产品和食品中具有应用.
- 目前的生产方法缺乏关于最佳微生物宿主和碳来源的明确指导,以实现商业可行性.
- 对技术经济和生命周期影响的有限理解阻碍了大规模的印地基因生产.
研究的目的:
- 评估和比较不同微生物宿主在印地基因生产中的商业潜力.
- 评估印素制造的技术经济可行性和环境足迹.
- 确定优化印地戈丁生产以实现商业可行性的关键因素.
主要方法:
- 针对五种不同的微生物宿主,利用了基因组规模的代谢模型.
- 综合技术经济评估 (TEA) 和生命周期评估 (LCA) 模型.
- 分析了使用葡萄糖,西洛斯和木质素衍生的芳香碳来源的生产.
主要成果:
- 与其他宿主和合成印花生产相比,Pseudomonas putida表现出优越的性能.
- 通过P. putida生产的印地基丁,实现了2.9美元/千克的最低销售价格,温室气体排放量为3.5千克CO2e/千克.
- 将路径优化到90%的理论收益率可以将成本降低6-7倍,温室气体排放量降低3-10倍.
结论:
- P. putida 是一个有前途的宿主,用于成本效益和可持续的印地基素生产.
- 芳香物的共同利用和共同增值分子的生产提高了经济和环境绩效.
- 全系统的改进和可持续的源对于商业化印地基丁生产至关重要.
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