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库库比特[7]uril-Styryl染料复合物用于DNA和细胞成像的光识别

Ekaterina Y Chernikova¹, Maria A Ustimova¹, Yuri V Fedorov¹

¹Laboratory of Photoactive Supramolecular Systems, A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Russia, 119991, Moscow, Vavilova St. 28.

Chembiochem : a European journal of chemical biology

|March 7, 2025

概括

新型的氨酸染料表现出不同的DNA结合模式,包括沟结合和间隙结合. 用CB封装[7]出乎意料地改变了染料-DNA相互作用,影响了潜在的药物输送和成像应用的聚合和细胞染色.

科学领域:

药用化学医学化学
超分子化学超分子化学
生物物理化学生物物理化学

背景情况:

滴定氨酸染料正在探索其在生物应用中的潜力.
了解配体-DNA相互作用对于开发向疗法和成像剂至关重要.

研究的目的:

研究具有不同终端组的新型二化染料的DNA结合特性.
检查合成分子容器CB[7]对染料-DNA相互作用的影响.
评估自由和封装染料的细胞毒性和细胞染色能力.

主要方法:

合成和表征二化色素的染料.
使用技术来确定沟结合和间隙结合的DNA结合研究.
在CB的存在下对染料-DNA相互作用的研究[7].
细胞毒性测定和活细胞成像.

主要成果:

染料1具有灵活的末端组,主要与DNA槽结合.
染料2具有刚性终端单元,具有双重结合模式:间隙结合和槽结合.
CB [7]封装意外导致DNA框架内部分染料保留,尽管CB [7]亲和力更高.
染料1在CB[7]-染料-DNA三元系统中形成左侧聚合物;染料2显示可逆间隙和轻微的右侧聚合.
自由染料和封装染料都表现出细胞染色能力和不同的细胞毒性.

结论:

氨酸染料的结构变化显著影响它们的DNA结合模式.
CB[7]可以调节染料-DNA相互作用,从而导致复杂的超分子组合.
这些发现为设计先进的药物递送系统和细胞成像/治疗平台提供了对联体-DNA相互作用的见解.

关键词:

它们是DNA DNA DNA DNA.细胞成像细胞成像库库尔比图里尔是一种.酸盐是一种酸盐.超分子化学超分子化学

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