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Updated: May 23, 2025

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

在大米土壤微观世界中的高N2O度下激活的贝塔蛋白细菌类II nosZ

Kazumori Mise¹, Yoko Masuda^2,3, Keishi Senoo^2,3

¹National Institute of Advanced Industrial Science and Technology, 2-17-2-1 Tsukisamu-higashi, Toyohira-ku, Sapporo, Hokkaido 062-8517, Japan.

Journal of applied microbiology

|March 7, 2025

概括

含有 nosZ 基因的贝塔蛋白菌有效地减少了土中的氧化 (N2O). 这些微生物显示出显著的N2O缓解潜力,为生物刺激策略提供了目标.

科学领域:

微生物学微生物学
环境科学环境科学
土壤科学土壤科学

背景情况:

米土壤微生物群落可以消耗氧化 (N2O),这是一个强大的温室气体.
了解负责减少N2O的特定微生物对于缓解策略至关重要.
在淹没的田地土壤中直接测量N2O减少是具有挑战性的.

研究的目的:

确定负责米土壤中N2O缓解能力的微生物群.
调查特定的氧化减少酶基因 (nosZ) 在N2O消耗中的作用.
评估贝塔蛋白细菌作为N2O吸收器的潜力.

主要方法:

用外部N2O修改的土微观宇宙的构建.
同时评估nosZ基因转录和N2O消耗.
转录基因测序用于分析微生物群落和基因表达.

主要成果:

外部N2O在微观宇宙中在6-8天内被大量消耗.
在大多数土壤中,贝塔蛋白细菌 nosZ,特别是 II 类 (Rhodocyclales,Nitrosomonadales) 主导 nosZ 转录 (> 50%).
公开可用的元基因组和16S rRNA数据显示,这些贝塔蛋白质细菌的存在无处不在,但并不占主导地位.

结论:

含有II nosZ分类的贝塔蛋白菌是强大的N2O降低剂,并在米土壤中起到显著的N2O沉降作用.
这些贝塔蛋白细菌代表了生物刺激的有希望的目标,以增强N2O缓解.
需要进一步的研究来充分阐明这些减少N2O的细菌的生态生理学.

关键词:

生物地质化学循环的过程.这就是meta-omics.的转化的转化土壤微生物生态学

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