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相关概念视频

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

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In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
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Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

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The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
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Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
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Activation of Integrins01:15

Activation of Integrins

3.2K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
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Integrins01:10

Integrins

3.7K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
3.7K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

6.9K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
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相关实验视频

Updated: May 23, 2025

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
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Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

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可调整的整合素 - 连接器合强度调节细胞自适应机制感应.

Zheng Zhang1,2,3, Xiaoxi Liu1,2, Baoyong Sha4

  • 1The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.

Nano letters
|March 7, 2025
PubMed
概括

细胞通过调节整合素-连接体相互作用来适应机械力. 这项研究揭示了DNA结合物如何影响细胞粘附和机械感知,影响生物材料设计.

关键词:
在RGD合器的强度强度.细胞粘附 细胞粘附细胞迁移 细胞迁移细胞-ECM相互作用机械传导 机械传导

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Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion
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A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro
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相关实验视频

Last Updated: May 23, 2025

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
07:55

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

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Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion
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A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro
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A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

Published on: August 27, 2015

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科学领域:

  • 细胞机械生物学 细胞机械生物学
  • 生物材料科学是生物材料的科学.
  • 分子细胞生物学分子细胞生物学

背景情况:

  • 细胞通过整体因子与细胞外基质 (ECM) 相互作用,施加引力.
  • 整合素-连接体结合对于细胞粘附和机械感知至关重要.
  • 粘附分子的张力耐受性影响细胞行为.

研究的目的:

  • 为了研究整合素-连接体合强度在细胞自适应机械感知中的作用.
  • 探索不同的DNA结合物 (dsDNA与slDNA) 如何在紧张状态下影响细胞粘附.
  • 了解合强度对细胞迁移 (durotaxis) 的影响.

主要方法:

  • 对Arg-Gly-Asp (RGD) 与dSDNA和slDNA结合物的共价结合.
  • 在PEG基板上的DNA-RGD结的固定.
  • 测量细胞对不同紧张的反应,包括actin聚合和cofilin酸化.

主要成果:

  • 干环DNA (slDNA) 连接器在高张力下保持结合,与双链DNA (dsDNA) 连接器不同.
  • 细胞通过调节行为因子动态和cofilin酸化来适应粘附,以保持整合素-联结体合.
  • 整合素-连接体合强度决定了细胞是否表现出正或负的durotaxis.

结论:

  • 整合素-连接体合强度是细胞自适应机械感知的一个关键媒介.
  • 可调节的DNA带为设计具有受控粘合性质的生物材料提供了洞察力.
  • 了解这些机制对于基于细胞的应用和生物材料开发至关重要.