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Updated: May 23, 2025

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

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在Cyanogramide生物合成中的N-甲基转移酶CyaF的生物化学和结构见解

Ruijie Chen1,2, Qingbo Zhang1,2,3, Liping Zhang1,2,3

  • 1State Key Laboratory of Tropical Oceanography, Key Laboratory of Tropical Marine Bioresources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, People's Republic of China.

Journal of natural products
|March 7, 2025
PubMed
概括

研究人员发现了酶CyaF,对天然产品生物合成至关重要. 这种酶在β-carbolines中甲基化,对各种基质具有灵活性,在生物催化中具有潜力.

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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies10:01

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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

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Protein Organization01:24

Protein Organization

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Gene Families01:57

Gene Families

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Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Proteomics

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Protein Folding

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Structure of Porins

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