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改善对称性不匹配的大分子组合的冷EM图:关于鞭毛运动器的案例研究

Prashant K Singh1, T M Iverson2

  • 1Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA.

Journal of structural biology
|March 7, 2025
PubMed
概括

粒子减去对于解决具有对称性不匹配的复杂生物分子结构至关重要,比如细菌鞭毛电机. 这种方法显著提高了复杂分子组件的冷电子显微镜分辨率.

关键词:
在C环中,使用C环.低温电子显微镜的使用方法化EMEM 在冷EMEM.羽翼翼发动机的发动机.聚焦的精炼 聚焦的精炼地方提炼 地方提炼在MS-ring中使用.宏分子复合体是什么?方法 方法 方法微粒减法 微粒减法 微粒减法立体测量异质性的异质性对称性不匹配的不匹配.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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