用Corynebacterium glutamicum进行代谢工程,用于高度选择性地生产5-基酸
Yu Jung Sohn1, Hee Taek Kim2, Minsoo Kang3
1Department of Chemical Engineering and Materials Science, Graduate Program in System Health Science and Engineering, Ewha Womans University, Seoul, 03760, Republic of Korea.
Metabolic engineering
|March 7, 2025
概括
研究人员对Corynebacterium glutamicum进行了工程设计,以生产高产的5-基酸 (5-HV). 这种可持续的微生物工艺实现了88.23g/L的5-HV,最大限度地减少了δ-黄 (DVL) 合成的副产品.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 通过通过l-lysine降解从葡萄糖中生物合成的5-hydroxyvaleric酸 (5-HV) 产生了像l-lysine,5-aminovaleric酸 (5-AVA) 和谷氨酸 (GTA) 这样的副产品.
- 在Corynebacterium glutamicum中实现5-HV生产的高选择性是具有挑战性的,因为代谢途径相互连接.
研究的目的:
- 在Corynebacterium glutamicum中为5-HV开发一种高度选择性的微生物生产系统.
- 为了使得从微生物产生的5-HV.中能够可持续合成δ-valerolactone (DVL).
主要方法:
- 通过删除GTA生物合成途径并将GTA循环回收途径纳入工程Corynebacterium glutamicum.
- 通过删除l-lysine出口基因 (lysE) 并集成一个l-lysine转换模块,进一步修改了菌株.
- 使用料批发发酵来生产高度的5-HV,然后用酸处理来合成DVL.
主要成果:
- 最终的工程菌株获得了88.23g/L的5-HV标位,并显著减少了副产品 (3.28g/LGTA,1.16g/L5-AVA,没有可检测的l-lysine).
- 微生物产生的5-HV通过酸性处理以65%的效率被转化为δ-valerolactone (DVL).
- 证明了为5-HV和DVL生产提供高度选择性和可持续的平台.
结论:
- 代谢工程策略,包括基因删除和途径集成,协同增强了5-HV的选择性和产量.
- 开发的系统为生产DVL提供了一个环保和可持续的途径,DVL是一种有价值的工业C5溶剂.
- 这项工作为生物基生产C5化学品提供了一个强大的微生物平台.
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