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Updated: May 23, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

基于全转录基因组测序的识别和功能预测ZM-4 (Malus zumi) 中与盐耐受性相关的圆形RNA

Zhao Liu¹, Xiang Lu¹, Hanxin Guo²

¹College of Agriculture, Shihezi University, Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Shihezi, Xinjiang 832000, China; Research Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Xingcheng, Liaoning 125100, China.

International journal of biological macromolecules

|March 7, 2025

概括

盐应激会影响果根茎circRNAs (循环RNAs). 这项研究在耐盐性ZM-4中确定了新型circRNA和调节网络,揭示了耐盐性机制.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
无生物应激反应的应激反应

背景情况:

循环RNAs (circRNAs) 在植物非生物应激反应中至关重要.
环RNAs在果根茎的盐分耐受性中的作用在很大程度上仍未被探索.
了解这些机制对于开发有弹性的作物品种至关重要.

研究的目的:

在盐应激下研究耐盐 (ZM-4) 和盐敏感 (M9T337) 果根茎中的circRNA表达特征.
确定差异表达的circRNA及其在盐分耐受性中的潜在作用.
阐明涉及circRNAs,miRNAs和mRNAs的调节网络.

主要方法:

在NaCl压力下,从果根茎叶子和根茎中获得的circRNAs的比较转录组分析.
使用生物信息学识别和量化circRNAs.
对circRNA-miRNA-mRNA相互作用的分析和对父母基因的丰富分析.

主要成果:

确定了2502个circRNA,在两种根茎类型中都发现了独特的circRNA.
在ZM-4中差异表达的circRNA与代谢和二次代谢物生物合成途径有关.
构建了潜在的ceRNA网络,突出了通过miRNA调节盐敏感基因 (例如SPL6,COBL7) 的特定circRNA.

结论:

这项研究提供了关于果根茎盐耐受性的circRNA调节的基本理解.
已识别的circRNA和调节途径为改善果育种中盐分耐受性提供了目标.
循环RNA在果根茎中对盐应激反应的转录后调节中发挥着重要作用.

关键词:

马卢斯·祖米 (Malus zumi) 是一个非常糟糕的语言.盐的压力是盐的压力.环RNA 环RNA 是一个环RNA.

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