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相关实验视频

Updated: May 23, 2025

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

评估使用纳米孔测序对初级人体组织的DNA甲基化检测.

Rylee Genner^1,2, Stuart Akeson³, Melissa Meredith⁴

¹Center for Alzheimer's and Related Dementias, National Institute on Aging and National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.

Genome research

|March 7, 2025

概括

与R9相比,新的纳米孔R10测序化学显示了DNA甲基化检测的高度一致性,促进了大规模的基因组研究. 这一进步有助于在不同纳米孔化学物质之间进行比较.

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Targeted DNA Methylation Analysis by Next-generation Sequencing

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相关实验视频

Last Updated: May 23, 2025

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

科学领域:

基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
生物信息学是一种生物信息学.

背景情况:

DNA甲基化,主要是5-甲基 (5mC),在人类基因组中至关重要,与疾病有关.
单分子测序 (牛津纳米孔技术 [ONT],太平洋生物科学 [PacBio]) 允许本地DNA分析,包括甲基化.
ONT的R10化学升级承诺更高的准确性和吞吐量,但其对5mC检测的影响尚不清楚.

研究的目的:

在ONT R9和R10化学之间以纳米孔为基础的5mC检测的差异的计算特征.
通过使用各种人类基因组数据集,评估R9和R10之间的5mC调用一致性.
评估化学差异对甲基化分析工具的影响.

主要方法:

来自ONT R9和R10测序数据的5mC呼叫的计算分析.
在三个人类基因组数据集中比较甲基化检测:细胞系,大脑和血液.
对CpG岛屿和同聚合物区域进行深入分析,以获得甲基化精度.

主要成果:

在所有数据集中,纳米孔R9和R10化学物质之间观察到5mC检测的高一致性.
甲基化检测的最强相关性是纳米孔R10和Illumina双硫酸盐测序之间的细胞系数据.
化学品之间的甲基化数据的微妙差异可以影响下游分析工具,如差异甲基化调用器.

结论:

来自不同化学的纳米孔甲基化数据之间的比较是可行的指导假设.
这项研究为协调使用不同纳米孔化学物质的大规模测序工作数据提供了必要的见解.
这些发现支持纳米孔R10在大型基因组计划中可靠的5mC检测的实用性.