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Updated: May 23, 2025

An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis
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改进传输电子显微镜的样本制备:Trypan蓝色染色和低点合剂嵌入超薄细胞段

Noriyuki Ishii1, Yoshito Koja2, Keiichi Noguchi3

  • 1Cellular and Molecular Biotechnology Research Institute, Department of Life Science and Biotechnology, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central-6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan; Electron Microscopy Facility, Open Research Facilities Station, Open Research Platform Unit, Tsukuba Innovation Arena (TIA) Central Office, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central-6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan; The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan; Department of Physics, Hamamatsu University School of Medicine, 1-20-1 Handayama, Chuo, Hamamatsu, Shizuoka 431-3192, Japan.

Journal of bioscience and bioengineering
|March 7, 2025
PubMed
概括

研究人员通过使用Trypan Blue染色和低点 agar来改进传输电子显微镜 (TEM) 样品制备. 这种方法增强了视觉跟踪,避免了危险的化学物质,使得TEM样本处理更有效和更安全.

关键词:
没有膜的器官.阶段分离 阶段分离 阶段分离原始塑料是原始塑料中的一个.传输电子显微镜的使用特里潘蓝色的蓝色是什么意思超薄的截面是超薄的截面.

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