严重创伤后肠道微生物群和炎症性细胞因子的多样性
Chang-Qing Liu1,2, Jie Yang3, Hong-Fei Ren4
1Department of Operating Room of West China Hospital/West China School of Nursing, Sichuan University, No. 37, Guoxue Alley, Wuhou District, Chengdu, 610041, Sichuan, China.
Scientific reports
|March 7, 2025
概括
严重的创伤改变了肠道微生物群的多样性,增加了Muribaculaceae,同时减少了Prevotella. 准这些微生物变化可能有助于调节炎性细胞因子并加速恢复.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 创伤研究 创伤研究
背景情况:
- 肠道微生物群在严重创伤后果中起着至关重要的作用.
- 肠道微生物群影响炎症和创伤后恢复的具体机制尚未完全理解.
研究的目的:
- 为了研究肠道微生物组成的动态变化和严重压碎和骨折创伤后大鼠的炎症性细胞因子水平.
- 探索特定肠道细菌和炎症标志物之间的相关性,以了解它们在创伤反应和恢复中的作用.
主要方法:
- 建立一个严重的粉碎和骨折创伤的老鼠模型.
- 16S rRNA高通量基因测序用于分析肠道微生物群组成.
- 与酶相关的免疫吸收试验 (ELISA) 用于量化炎症性细胞因子 (IL-1α,IL-6,IL-8,TNF-α).
- 微生物种类和细胞因子水平之间的相关性分析.
主要成果:
- 严重的创伤导致了整体肠道微生物群的多样性丧失,并导致了Muribaculaceae的扩张.
- 普雷沃特拉和阿洛普雷沃特拉在创伤后表现出大量减少.
- 炎症性细胞因子水平 (IL-1α,IL-6,IL-8,TNF-α) 最初下降,然后在一周内增加.
- 在Prevotellaceae_UCG_001和TNF-α,Lactobacillus和IL-6/IL-1α/IL-8以及Lachnospiraceae_NK4A136_group/Muribaculaceae和IL-8之间发现了特定的相关性.
结论:
- 严重的创伤会引发压力反应,从而对肠道微生物群产生负面调节,并影响炎症性细胞因子配置.
- 调节特定的肠道细菌和维持炎症性细胞因子平衡是加速严重创伤后恢复的潜在策略.
相关概念视频
01:23Anatomy of the Intestines
71.2K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
71.2K
01:17Irritable Bowel Syndrome I: Introduction
215
Irritable Bowel Syndrome (IBS) is characterized by functional disturbances in the gastrointestinal system, presenting a cluster of symptoms without evident structural or biochemical abnormalities. It primarily affects the large intestine and may cause abdominal pain, bloating, excessive gas, diarrhea, constipation, or both.
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
215
01:17Chronic Bowel Disorders: Introduction
375
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
375
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
108
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
108
01:29Bacterial Flora of the Large Intestine
370
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
370
01:30Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
291
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
291