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在Monilinia真菌植物病原体和TE驱动的DMI抗性适应基因组结构中的可转移元素

Muhammed Raşit Durak^1,2, Hilal Özkılınç^3,4

¹School of Graduate Studies, M.Sc. Program in Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, Çanakkale, 17100, Türkiye.

|March 7, 2025

概括

可移植元素 (TE) 与菌剂耐药性有关. 在CYP51基因上游的一个特定的TE降低了对普罗比可纳的敏感性,影响了疾病管理策略.

科学领域:

植物病理学植物病理学
菌类基因组学菌类基因组学
分子进化的分子进化.

背景情况:

植物病原体对真菌杀菌剂的耐药性是一个重大挑战.
莫尼利尼亚物种是研究真菌病理系统的关键植物病原体和模型.
脱甲基化抑制剂 (DMI) 杀菌剂,如普罗比可纳,对于疾病控制至关重要.

研究的目的:

调查可转移元素 (TE) 动态在Monilinia fructicola和Monilinia laxa中对DMI-propiconazole耐药性的作用.
描述在选择性压力下的 TE 含量和演变.

主要方法:

在129个莫尼利尼亚分离物中评估了普罗比可纳的敏感性.
进行了全面的TE识别和量化.
利用实验进化框架研究在杀菌剂压力下的基因组变化.
分析了CYP51的基因表达.

主要成果:

测试特异体约占蒙尼利尼亚基因组的9%,其中I类元素占主导地位.
在康纳压力下的实验进化导致TE含量发生动态变化,观察到整体扩张.
在CYP51基因上游的嵌套TE与M. fructicola. 葡萄糖中降低了对普罗比可纳的敏感性有关.
在含有TE和缺乏TE的分离物之间,CYP51基因表达有显著差异.

结论:

确立了TEs和DMI真菌杀菌剂耐药性之间的直接联系.
为了研究基因组适应,开发了一种新的实验进化系统.
这些发现为改善疾病管理提供了对分子耐药机制的洞察.

关键词:

莫尼利尼亚物种 (Monilinia) 的物种.实验进化的实验进化.抗真菌杀菌剂的耐药性抗真菌杀菌剂的耐药性可转移的要素是可以转移的.

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