相关实验视频
Updated: Jun 28, 2026

08:03
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
Published on: April 11, 2017
9.5K
circ_0000389通过准miR-346/KLF7轴来抑制椎间盘变性
Chun Liu1, Cheng Yu1, Yang Duan1
1Department of Spinal Surgery, Orthopedic Medical Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Acta biochimica et biophysica Sinica
|March 8, 2025
概括
循环RNAcirc_0000389在椎间盘退化 (IVDD) 中是下调的. 它通过调节miR-346/KLF7轴来抑制细胞外基因分解,为IVDD提供了潜在的治疗标.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 整形外科 整形外科 整形外科
背景情况:
- 椎间盘退化 (IVDD) 是腰部疼痛的主要原因.
- 循环RNAs (circRNAs) 越来越多地被认为在IVDD进展中的作用.
- 在IVDD中circ_0000389的特定功能仍然在很大程度上是未知的.
研究的目的:
- 调查circ_0000389在IVDD进展中的作用和机制.
- 为了确定IVDD治疗的潜在治疗点.
主要方法:
- 对GSE67566数据集的生物信息分析以确定circ_0000389.
- 通过RT-qPCR测量核脉 (NP) 组织和细胞中的基因表达.
- CCK-8测定用于评估细胞增殖.
- 西方斑点用于分析细胞外基质 (ECM) 代谢.
- 双 luciferase 记者基因测定和 RNA 免疫沉 (RIP) 来确认分子相互作用.
主要成果:
- 在退化的NP组织中,Circ_0000389的表达显著下调.
- 发现Circ_0000389可以抑制核细胞 (NPC) 中的ECM代谢.
- 该研究证实circ_0000389与miR-346相互作用,miR-346针对KLF7,建立了circ_0000389/miR-346/KLF7轴.
结论:
- Circ_0000389通过抑制ECM代谢,在IVDD中起着保护作用.
- 环_0000389/miR-346/KLF7轴是IVDD进展中的关键调节途径.
- Circ_0000389代表了管理IVDD的有希望的治疗目标.
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...

