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重编程的糖解诱导增强NIR-II激发光动力学/光热疗法

Chunbai Xiang¹, Qihang Ding², Ting Jiang³

¹Guangdong Key Laboratory of Nanomedicine, CAS-HK Joint Lab for Biomaterials, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University Cheng Du 610064 China.

|March 8, 2025

概括

这项研究引入了一种新的多功能光敏感剂 (PPQ-CTPA) 和一种针对线粒体的药物 (LND-TPP),用于联合光动力学疗法 (PDT) 和光热疗法 (PTT). 封装在纳米粒子中,它们通过近红外II激光激活有效地抑制小鼠的乳腺癌生长.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
癌症治疗方法癌症治疗方法

背景情况:

小分子光学材料为先进的诊断和治疗提供可调节性质和生物相容性.
结合光动力疗法 (PDT) 和光热疗法 (PTT) 的多功能药物显示出增强癌症治疗的前景.
近红外II (NIR-II) 激发 (>1000nm) 能够更深入地穿透组织,用于体内成像和治疗.

研究的目的:

为了合成和描述一种新型的多功能光敏感剂 (PPQ-CTPA),用于联合PDT和PTT.
开发一种针对线粒体的药物 (LND-TPP),以增强治疗疗效.
创建一个纳米粒子配方 (PPQ-CTPAL NPs) 用于协同的癌症治疗,并评估其体内性能.

主要方法:

合成PPQ-CTPA和LND-TPP,然后封装到DSPE-SS-PEG中形成PPQ-CTPALNP.
评估PPQ-CTPAL NP通过谷氨 (GSH) 的分解和活性成分的释放.
在体外评估对乳腺癌细胞系的治疗效果.
在体内研究中,使用乳腺癌小鼠模型,对PDT和PTT进行1064nm激光照射.

主要成果:

在NIR-II (1064 nm) 激发下,PPQ-CTPA有效地产生反应性氧物种 (ROS) 和热能.
LND-TPP抑制了线粒体糖解和HSP 90,增强了PDT和PTT的影响.
在癌细胞中,PPQ-CTPAL NP显示了PPQ-CTPL和LND-TPP的触发释放.
在接受PPQ-CTPALNP和1064nm激光照射的小鼠中观察到显著的瘤生长抑制.

结论:

PPQ-CTPAL NPs为联合PDT,PTT和化疗提供了一个协同平台.
开发的纳米颗粒显示出有效治疗乳腺癌的巨大潜力.
这种方法突显了多功能光疗剂在NIR-II辐射下临床适用性.

关键词:

葡萄糖溶解抑制的抑制作用轻度 PTT 的情况.多模式成像技术多模式成像技术在NIR-II光激发过程中,第一种类型的PDT.

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