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生理学分析和转录基因组测序显示,HSPA1参与厚贝,Mytilus coruscus中热应激反应

Huajian Lin¹, Ronghui Yao¹, Sisi Wei¹

¹Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316022, PR China.

Comparative biochemistry and physiology. Part D, Genomics & proteomics

|March 8, 2025

概括

Mytilus coruscus使用热冲击蛋白1 (HSPA1) 来保护自己免受热应激损伤. 这项研究揭示了HSPA1在缓解细胞损伤和细胞亡方面的关键作用,为双适应提供了洞察力.

科学领域:

海洋生物学海洋生物学
分子生物学分子生物学
环境压力生理学生理学

背景情况:

Mytilus coruscus对温度波动表现出敏感性的表现.
热应激可以诱导贝的组织损伤和亡.
在热应激下,热冲击蛋白1 (HSPA1) 表达的调节机制需要进一步阐明.

研究的目的:

为了研究高温压力下Mytilus coruscus中HSPA1基因表达的调节.
了解HSPA1在减轻热引起的细胞损伤和细胞亡中的作用.
探索HSPA1在抗氧化剂反应途径中的参与.

主要方法:

在Mytilus coruscus中诱导热应激.
过度表达和击倒HSPA1基因.
组织损伤和亡的评估.
对抗氧化酶的酶活性测定 (SOD,CAT,GSH-PX).
转录组分析以确定差异表达的基因和途径.

主要成果:

高温导致M. coruscus. 的组织损伤和亡.
过度表达HSPA1减轻了热引起的损伤,并增强了抗氧化酶活性.
在热应激下对HSPA1的抑制导致关键基因的表达增加 (HSPA1S,HSP90,HSPA5,DnaJA1,JUN).
在MAPK信号传递,ER蛋白处理,TNF信号传递和亡途径中,差异性基因表达得到了丰富.

结论:

通过减轻细胞损伤和亡,HSPA1在保护Mytilus coruscus免受高温压力的过程中发挥着至关重要的作用.
抗氧化反应和特定的信号通路是贝热应激防御的重要组成部分.
这些发现提供了对面对环境温度变化的双动物物种的保护和适应性演变的见解.

关键词:

热应激是一种热应激.这种植物是Mytilus coruscuscus.身体生理学身体生理学在RNA-seqqq.dsRNARNA是什么意思

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